人脐带羊膜来源和骨髓来源间充质干细胞的免疫调控特征比较  

Comparison of Immunologic Regulatory Characteristics of Mesenchymal Stem Cells Derived from Human Umbilical Cord Amnion and Adult Bone Marrow

作　　者：徐曼[1] 刘元林[1] 陈晨[1] 廖丽[2] 张毅[1] 陈虎[2] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850 [2]军事医学科学院附属医院,北京100071

出　　处：《中国实验血液学杂志》2011年第5期1224-1229,共6页Journal of Experimental Hematology

基　　金：十一五新药创制重大专项(编号2009ZX09503-23);国家863重大专项课题(编号2006AA02Z4A0);国家自然科学基金(编号31070996)

摘　　要：本研究旨在比较人脐带羊膜来源的间充质干细胞(mesenchymal stem cells,MSC)与骨髓来源MSC的免疫调控能力,为临床开展异基因造血干细胞移植提供实验依据。采用胶原酶消化法分离人脐带羊膜贴壁细胞,并从细胞形态、生长特性、细胞表型、多向分化能力进行鉴定。利用淋巴细胞转化实验和混合淋巴细胞反应比较人脐带羊膜来源MSC与人骨髓来源MSC的免疫调控能力的差异。结果表明,人脐带羊膜来源和骨髓来源的MSC具有相似的生物学特征,即细胞呈成纤维样形态生长,并具有强大的体外扩增能力;流式细胞仪检测细胞表型结果显示,脐带羊膜来源MSC高表达CD73、CD90、CD105,而骨髓来源MSC表面标志CD34、CD45以及参与免疫反应的细胞表面分子HLA-DR和CD86等为阴性。其诱导多向分化能力结果表明,两组细胞均可向成脂肪、成骨和成软骨分化。混合淋巴细胞反应和淋巴细胞转化实验证明,两组MSC均可抑制异体T细胞的增殖,且随MSC数量的增加,抑制效应逐渐增强;RT-PCR检测显示两组MSC表达相同的免疫相关因子。结论:人脐带羊膜来源的MSC可以替代成人骨髓MSC,可作为满足实验和临床需要的MSC重要来源。This study was aimed to compare the immunoregulatory effects of mesenchymal stem cells derived from human umbilical cord amnion（AMSC） and adult bone marrow（BMMSC） in vitro,so as to provide the experimental basis for allogeneic hematopoietic stem cell transplantation in clinic.The AMSC were isolated from human umbilical cord amnion by using digestion with collagenase.They were identified by morphology,growth characteristics,immunophenotyping and differentiation ability.Furthermore,the immunoregulatory effects of AMSC and BMMSC were tested by lymphocyte transformation and mixed lymphocyte reaction.The results showed that AMSC and BMMSC possessed similar biological characterisitics such as exhibition of fibroblastic morphology and strong prolifiration ability in vitro.Flow cytometric analysis revealed that the AMSC highly expressed CD73,CD90,CD105,but negative for CD34,CD45,HLA-DR,and CD86 of BMMSC.Functionally,they all could differentiate into adipocyte,osteocytes and chondrocytes.Moreover,AMSC could inhibit cellular or nonspecific mitogenic stimuli-induced T cell proliferation with a dose-dependent manner.Reverse transcriptional-polymerase chain reaction also demonstrated expression of the similar immune cytokines in AMSC and BMNSC.It is concluded that the MSC derived from human umbilical cord amnion may be an excellent alternative source for experimental and clinical application.

关 键 词：间充质干细胞 脐带羊膜 骨髓 免疫调控 

分 类 号：R392.12[医药卫生—免疫学] R446.62[医药卫生—基础医学]