自身免疫性血小板减少性紫癜患者外周血中CD4^+ CD25^+调节性T细胞、sFas和sFasL的表达及临床意义  

Expression and Clinical Significance of CD4^+ CD25^+Treg Cells,sFas and sFasL in Peripheral Blood of Patients with Autoimmune Thrombocytopenic Purpura

作　　者：贾瑞萍[1] 赵雪芸[1] 

机构地区：[1]包头市中心医院血液科,内蒙古自治区包头014040

出　　处：《中国实验血液学杂志》2011年第5期1264-1267,共4页Journal of Experimental Hematology

摘　　要：本研究通过检测成人自身免疫性血小板减少性紫癜(AITP)外周血中CD4+CD25+调节性T细胞(Treg)、sFas和sFasL的表达,探讨它们在AITP发病机制中的作用及临床意义,为寻求AITP治疗的有效方法提供理论依据。采用流式细胞术分别检测30例AITP患者和18例正常对照者外周血CD4+T、Treg、CD4+CD25-T细胞表达率及Treg/CD4+T比值;用酶联免疫吸附法(ELISA)检测AITP患者治疗前后及对照组外周血sFas、sFasL表达水平。结果表明,AITP组外周血中CD4+T细胞表达率低于对照组(p<0.05),Treg细胞表达率及Treg/CD4+T比值明显低于正常对照组(p<0.01)。AITP组患者治疗前外周血中sFas和sFasL水平明显高于治疗后和正常对照组(p<0.01),AITP组治疗后与正常对照组外周血sFas、sFasL水平差异无统计学意义(p>0.05)。AITP患者治疗前Treg细胞表达率、Treg/CD4+T细胞比值与血小板计数呈正相关;AITP患者外周血中sFas和sFasL水平呈正相关;CD4+T细胞、CD4+CD25-T细胞表达率,sFas、sFasL浓度与血小板计数无明显相关;Treg细胞表达率和sFas、sFasL浓度间没有明显相关性。结论:Treg在AITP的发病机制中发挥一定作用;Treg细胞水平与AITP病情的严重程度有关;sFas和sFasL水平异常参与了AITP的免疫病理过程。This study was aimed to detect the expression of CD4＋CD25＋ regulatory T cells（Treg）,sFas and sFasL in patients with autoimmune thrombocytopenic purpura（AITP）,and to explore their roles in the pathogenesis of AITP and clinical significance,so as to provie a theoretical basis for effective treatment for AITP.The expressions of CD4^＋T,Treg,CD4^＋CD25-T,Treg /CD4^＋T in peripheral blood of 30 the patients with AITP and 18 controls were detected by flow cytometry,and enzyme-linked immunosorbent assay（ELISA） was used to detect the levels of sFas and sFasL in peripheral blood of controls and the patients with AITP before and after treatment.The results indicated that the expression rate of CD4^＋T in AITP patients was lower than that in the control（p0.05）,and the expression rates of Treg and Treg/CD4^＋T were significantly lower than those of control（p0.01）,but the expression rate of Treg between the two group had no difference（p0.05）.The levels of sFas and sFasL in the peripheral blood of the patients before treatment were significantly higher than that after treatment and control group（p0.01）,and no difference between the patients after treatment and the control group（p0.05） was found.The expression rates of Treg,Treg /CD4＋T were positively related with the platelet count and the level of sFas was positively related with the level of sFasL in the peripheral blood of AITP before treatment.There were no significant correlation between the levels of CD4＋T,Treg,sFas,sFasL and the platelet count.No correlation was seen between the expression of Treg and sFas,sFasL.It is concluded that CD4^＋CD25^＋ Treg play a role in the pathogenesis of AITP;the expression rate of Treg is associated with the severity of AITP;the abnormal levels of sFas and sFasL participate in the immune pathogenesis of AITP.

关 键 词：自身免疫性血小板减少性紫癜 CD4+ CD25+调节性T细胞 SFAS SFASL 

分 类 号：R554.6[医药卫生—血液循环系统疾病]