Morphological characteristics of the striatal neural pathway by biotinylated dextran amine tracing in rats  

作　　者：Bingbing Liu Shuhua Mu Lisi Ouyang Yaxi Zhu Keyi Li Mali Zhan Zongwei Liu Yu Jia Wanlong Lei 

机构地区：[1]Department of Anesthesiology, Guangdong Provincial Second People＇s Hospital, Guangzhou 510317, Guangdong Province, China [2]Department of Anatomy, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China

出　　处：《Neural Regeneration Research》2011年第25期1931-1936,共6页中国神经再生研究（英文版）

基　　金：the National Natural Science Foundation of China, No. 31070941; 20831006; 30770679

摘　　要：Using neural pathway tracing and immunohistochemical technique, the striato-direct pathway （BDA3 kDa injected into the rat lateral globus pallidus） and striato-indirect pathway （BDA3 kDa injected into the substantia nigra pars reticulata） neurons were specifically labeled, and then subjected to double-labeled immunohistochemistry for mu-OPIOID Receptor （specifically-labeled striatal patch compartment）, D1, and D2, respectively. The experimental findings showed that there are no statistically significant differences in the soma diameter and the number of primary dendrites between the striato-direct （substantia nigra pars reticularis） and indirect （globus pallidum externum） neurons labeled retrograde by BDA3 kDa. In addition, these two kinds of projection neurons revealed no obvious coexistence. This evidence indicates that as a highly sensitive neural pathway tracer, BDA could yield reliably and exquisitely detailed labeling of target neurons and synaptic structures. The variance of the morphologic structures and the localization of neurons were not statistically significant between the striato-substantia nigra pars reticularis and the globus pallidum externum projection neurons. Mesencephalic and thalamic neurons correlated with striatal neurons in morphology. Especially the latter which make typical excitatory synaptic contacts with striato-direct and -indirect neurons. Thus, this evidence suggests that thalamic neurons may extensively excite striatal neurons.Using neural pathway tracing and immunohistochemical technique, the striato-direct pathway （BDA3 kDa injected into the rat lateral globus pallidus） and striato-indirect pathway （BDA3 kDa injected into the substantia nigra pars reticulata） neurons were specifically labeled, and then subjected to double-labeled immunohistochemistry for mu-OPIOID Receptor （specifically-labeled striatal patch compartment）, D1, and D2, respectively. The experimental findings showed that there are no statistically significant differences in the soma diameter and the number of primary dendrites between the striato-direct （substantia nigra pars reticularis） and indirect （globus pallidum externum） neurons labeled retrograde by BDA3 kDa. In addition, these two kinds of projection neurons revealed no obvious coexistence. This evidence indicates that as a highly sensitive neural pathway tracer, BDA could yield reliably and exquisitely detailed labeling of target neurons and synaptic structures. The variance of the morphologic structures and the localization of neurons were not statistically significant between the striato-substantia nigra pars reticularis and the globus pallidum externum projection neurons. Mesencephalic and thalamic neurons correlated with striatal neurons in morphology. Especially the latter which make typical excitatory synaptic contacts with striato-direct and -indirect neurons. Thus, this evidence suggests that thalamic neurons may extensively excite striatal neurons.

关 键 词：neural pathway tracing rat striato immunoelectron microscopy direct pathway indirect pathway 

分 类 号：Q959.530.5[生物学—动物学] Q426