Screening proteins that interact with mutant superoxide dismutase 1 from familial amyotrophic lateral sclerosis using a yeast two-hybrid system  

作　　者：Guisheng Chen Shugui Shi Lusi Li Kangning Chen Ju HU Zhenhua Zhou Jun WU GaoxingLuo ShunzongYuan Xu Peng 

机构地区：[1]Department of Neurology, Ningxia Medical University General Hospital, Yinchuan 750004, Ningxia Hui Autonomous Region, China [2]Department of Neurology, Southwest Hospital, Third Mifitary Medical University of Chinese PLA, Chongqing 400038, China [3]Research Institute of Burn, Southwest Hospital, Third Military Medical University of Chinese PLA, Chongqing 400038, China

出　　处：《Neural Regeneration Research》2011年第26期2013-2017,共5页中国神经再生研究（英文版）

基　　金：the National Natural Science Foundation of China, No. 30300116

摘　　要：The present study screened a human fetal brain cDNA library to find the proteins that interact with mutant superoxide dismutase 1 （SOD1） using a yeast two-hybrid system. Using BLAST software, 15 real proteins which interacted with mutant SOD1 were obtained, including 8 known proteins （protein tyrosine-phosphatase non-receptor type 2, TBCl D4, protein kinase family, splicing factor, arginine/serine-rich 2, SRC protein tyrosine kinase Fyn, β-sarcoglycan; glycine receptor a2, microtubule associated protein/microtubule affinity-regulating kinase 1, ferritin H chain）, and 7 unknown proteins. Results demonstrated interaction of mutant SOD1 with microtubule associated protein/microtubule affinity-regulating kinase 1 and β-sarcoglycan.The present study screened a human fetal brain cDNA library to find the proteins that interact with mutant superoxide dismutase 1 （SOD1） using a yeast two-hybrid system. Using BLAST software, 15 real proteins which interacted with mutant SOD1 were obtained, including 8 known proteins （protein tyrosine-phosphatase non-receptor type 2, TBCl D4, protein kinase family, splicing factor, arginine/serine-rich 2, SRC protein tyrosine kinase Fyn, β-sarcoglycan; glycine receptor a2, microtubule associated protein/microtubule affinity-regulating kinase 1, ferritin H chain）, and 7 unknown proteins. Results demonstrated interaction of mutant SOD1 with microtubule associated protein/microtubule affinity-regulating kinase 1 and β-sarcoglycan.

关 键 词：yeast two-hybrid system mutant superoxide dismutase 1 cDNA library protein-protein interaction screen amyotrophic lateral sclerosis 

分 类 号：Q51[生物学—生物化学] Q554.9