逆转录酶活性检测方法在金黄仓鼠中的应用  

The Application of Reverse Transcriptase Assay in Golden Hamster

作　　者：付瑞[1] 巩薇[1] 岳秉飞[1] 贺争鸣[1] 

机构地区：[1]中国食品药品检定研究院,国家实验动物微生物、遗传检测中心,北京100050

出　　处：《实验动物与比较医学》2011年第5期381-383,共3页Laboratory Animal and Comparative Medicine

摘　　要：目的应用逆转录酶活性检测方法对金黄仓鼠及其它动物血清、细胞进行检测。方法根据雀麦草花叶病毒RNA序列设计一对引物，加入样品对RNA进行逆转录PCR，电泳观察有无特异条带以确定样品中是否含有逆转录酶。结果在兔、豚鼠、大鼠以及仓鼠血清中检出逆转录酶活性阳性样本。结论该方法具有良好的敏感性和特异性，可以应用于动物血清中逆转录酶活性的检测。Objective To detect the reverse transcriptase （RTase） activity in the serum of golden hamster, rabbit, guinea pig, rat, mouse, simian and in Veto, BHK21, PERV cell on the basis of RTase assay. Method A pair of primer is designed according to the BMV RNA sequence. As reverse transcfiptase, the serum and cells are tested by reverse transcript PCR （RT-PCR）, and the specific band is observed by electrophoresis. Results The RTase activity is positive in rabbit, guinea pig, rat and golden hamster serum. Conclusion This assay has fine specificity and sensitivity, and it can be used in detection for RTase activity in animal serum.

关 键 词：仓鼠 逆转录酶活性 逆转录PCR 

分 类 号：Q95-33[生物学—动物学]