碳化二亚胺交联去细胞猪肺动脉带瓣管道物理性能的研究  

Study on the Physical Characteristics of Decellularized Porcine Pulmonary Valved Conduits Crosslinked by Carbodiimide

作　　者：郭海平[1] 李温斌[2] 王圣[3] 史海峰[2] 郭俊平[1] 靳振生[1] 薛丽霞[1] 崔鹤仙[1] 梁建国[1] 

机构地区：[1]河北省邯郸市第一医院心胸外科,河北邯郸056002 [2]首都医科大学附属北京安贞医院心脏外科,北京100029 [3]河南省人民医院心脏外科,郑州450003

出　　处：《中国胸心血管外科临床杂志》2011年第5期447-452,共6页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

摘　　要：目的研究碳化二亚胺(carbodiimide,EDC)交联去细胞猪肺动脉带瓣管道体外的物理性能。方法在相对无菌条件下取得20个猪带瓣肺动脉,沿肺动脉瓣膜交界处纵向剪开分成3份(每份样本都由肺动脉管壁及其瓣膜组织组成)。将3份样本用抽签法随机分为3组:A组为对照组,为新鲜猪肺动脉带瓣管道样本,不再进一步处理;B组为去细胞组织样本,采用胰蛋白酶+去污剂Triton X-100对猪带瓣肺动脉样本进行去细胞处理;C组为EDC交联去细胞组织样本,即在B组处理的基础上进一步行EDC交联处理。测试肺动脉组织含水量、厚度、抗张强度和热皱缩温度,评价所有样本的物理性能。结果胰蛋白酶+去污剂能完全去除猪肺动脉带瓣管道组织的内膜层内皮细胞和管壁及瓣膜组织的细胞和部分基质成分,保留比较完整的纤维支架成份。与A组相比,B组肺动脉壁样本含水量增加(P=0.000),肺动脉瓣膜含水量变化不明显,肺动脉壁(P=0.000)和瓣膜(P=0.000)的厚度减少、抗张强度下降(P<0.01)、热皱缩温度无明显变化。与B组比较,C组肺动脉管壁样本含水量减少(P=0.000),肺动脉瓣膜样本含水量变化不明显。肺动脉管壁和瓣膜样本的厚度变化不明显、抗张强度增加(P<0.01)、热皱缩温度增加(P=0.000,0.000)。与A组相比,C组肺动脉壁和瓣膜含水量变化不明显,肺动脉壁和瓣膜的厚度减小(P=0.000,0.000),抗张强度无明显差别,热皱缩温度增加(P=0.000,0.000)。结论 EDC交联去细胞方法能够加强去细胞肺动脉带瓣管道的抗张强度、降低去细胞肺动脉管壁的含水量。Objective To observe the physical characteristics of decellularized porcine pulmonary valved conduits crosslinked by carbodiimide（EDC）. Methods Twenty porcine pulmonary valved arteries were mobilized on relative asepsis condition.They were cut longitudinally into three samples at the junction position of pulmonary valve（every sample was comprised of a part of the pulmonary conduit wall and the corresponding valve）.The samples were randomly divided into three groups by lot-drawing method.Group A was the control group which was made up of the fresh porcine arterial valved conduit samples without any other treatments.Group B was comprised of porcine pulmonary samples decellularized by trypsin-detergent digestion.Group C included the decellularized porcine pulmonary samples cross-linked by EDC.We observed the water content,thickness,tensile strength,and shrinkage temperature of all the samples,based on which the physical characeteristics of these samples were analyzed. Results Complete cell-free pulmonary conduit matrix was achieved by trypsin-detergent digestion.Compared with group A,in group B,the water content of pulmonary wall was significantly higher（P=0.000）,while the water content of pulmonary valve was not significantly different;the thickness of pulmonary wall and valve（P=0.000,0.000） and tensile strength of pulmonary wall and valve（P〈0.01） was significantly lower,while shrinkage temperature was not significantly different.Compared with group B,in group C,the water content of pulmonary wall was significantly lower（P=0.000）,while the water content of pulmonary valve,and the thickness of pulmonary wall and valve were not significantly different;the tensile strength of pulmonary wall（P〈0.01） and valve（P=0.000）,and the shrinkage temperature of them（P=0.000,0.000） were significantly higher.Compared with group A,in group C,the water content of pulmonary wall and valve,and the tensile strength of them were not statistically different,while the thickness of pulmonary wall and

关 键 词：碳化二亚胺 交联 去细胞 肺动脉 心脏瓣膜 

分 类 号：R318.08[医药卫生—生物医学工程]