干化学法检测血浆中的乙醇含量  被引量:4

Dry Chemical Assay of Plasma Levels of Ethanol

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作  者:敖家富[1] 石秀芳[1] 王祥英 

机构地区:[1]亳州市人民医院,安徽236800 [2]亳州市疾控中心,安徽236800

出  处:《安徽卫生职业技术学院学报》2011年第5期86-87,共2页Journal of Anhui Health Vocational & Technical College

摘  要:目的:快速、准确、方便检测血液乙醇含量。方法:采用干化学法检测血液乙醇含量(只需将血离心测血清或血浆)。结果:乙醇在线性范围0~320 mg/100ml内变异系数均小于5%,回收率95.8%~98.3%,与顶空气相色谱法相关系数为0.996。结论:干化学法操作简单、快速、方便、灵敏度高、标本用样量少、全自动化,精密度、回收率、重现性结果均满意。Objective: To detect plasma alcohol content fast,accuratly and easily.Method: Serum or plasma of patients was collected from peripheral blood samples by centrifugation.Ethanol content of Serum or plasma was detected with dry chemical method.Result: In the linear range of 0~320 mg/100 Ml,coefficient of variation of Ethanol were less than 5% and recovery was 95.8%~98.3%,and correlation coefficient was 0.996 between dry chemical method and headspace gas chromatography assay.Conclusion:Dry chemistry method is simple,fast,convenient,highly sensitive,fully automated and requires fewer specimens,and results of precision,recovery and reproducibility are satisfactory.

关 键 词:干化学法 快速准确 全自动化 乙醇含量 

分 类 号:R446.11[医药卫生—诊断学]

 

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