实时精子分离系统对猪精子冷冻品质的影响  

Effect of real-time sperm separation system on the quality of boar sperm frozen

作　　者：杨海[1,2] 李青旺[1] 胡建红[1] 张树山[3] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]衡阳师范学院生命科学系,湖南衡阳421008 [3]上海市农业科学院畜牧兽医研究所,上海201106

出　　处：《西北农林科技大学学报（自然科学版）》2011年第10期53-57,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家高技术研究发展计划(863)项目(2008AA101004)

摘　　要：【目的】探索实时精子分离系统对猪精子冷冻品质的影响。【方法】采用实时精子分离系统对精液进行分离优选,液氮冷冻法制作细管冻精,以未分离精液为对照,检测实时精子分离系统对猪精子冷冻品质的影响。【结果】分离优选精子解冻后,精子活率、顶体完整率、质膜完整率、正常染色质率均明显高于对照组(P<0.01);分离组精子的正常形态率也显著高于对照组,且未受冷冻-解冻过程的影响(P>0.01);分离过程对精子密度也未产生显著影响(P>0.01)。【结论】实时精子分离系统可以显著改善猪冷冻精液的品质。【Objective】 The study was to explore the effect of real-time sperm separation system on the quality of boar semen frozen.【Method】 Original semen was separated with real-time sperm separation system and tubule-froze with liquid nitrogen.The original semen was looked as control group,and the effect of real-time sperm separation system on the quality of boar semen frozen was detected.【Result】 After the separated semen was thawed,the sperm motility,the rate of spermatozoa with intact acrosome,the rate of spermatozoa with normal plasma membrane,the rate of spermatozoa with normal chromatin of the separated group were higher than those of the control group（P0.01）;For the rate of spermatozoa with normal morphology,the separated group was better than that of the control group（P0.01）,but it was not affected by the freeze-thaw process（P0.01）;The spermatozoa density was not significantly affected by the separation process（P0.01）.【Conclusion】 The real-time sperm separation system can significantly improve the quality of boar frozen semen.

关 键 词：实时精子分离系统 猪 精子 精液冷冻 精液品质 

分 类 号：S828.34[农业科学—畜牧学]