IL-28B遗传多态性与乙型肝炎病毒感染后病毒自然清除和疾病进展关系的研究  

Studies on the relationship between polymorphism of IL-28B rs8099917 and the outcome of HBV infection

作　　者：焦晓磊[1] 高英堂[2,3] 景丽[2] 刘彤[2] 石文霞[2] 郭华[2] 杨斌[2] 杜智[2] 

机构地区：[1]天津医科大学第三中心临床学院,300170 [2]天津市人工细胞重点实验室 [3]天津经济技术开发区企业博士后工作站天津市紫波高科技有限公司分站

出　　处：《中华流行病学杂志》2011年第11期1143-1147,共5页Chinese Journal of Epidemiology

基　　金：天津市应用基础及前沿技术研究计划（08JCYBJC08300）;天津市卫生局攻关项目（11KG112）

摘　　要：目的探讨IL-28B基因rs8099917位点（T〉G）单核苷酸多态性（sNP）与乙型肝炎病毒（HBV）感染后病毒自然清除和疾病进展的关系。方法采用TaqMan SNP基因分型的方法对健康人群（n=144）、感染HBV后白愈人群（n=143）、HBV持续感染人群（肝硬化患者100例，肝癌患者99例）进行IL-28B基因rs8099917位点的多态性检测。结果基因型和等位基因频率在各组及总体均符合Hardy．Weinberg遗传平衡。486例研究对象的基因型分布：TT型占89．3％、GT占10．5％、GG占0．2％；等位基因频率：T为94．5％，G为5．5％。该位点基因型和等位基因频率在各组间及各组内不同性别间的差异均无统计学意义（P〉0．05）。以健康组为对照经性别和年龄因素调整后，TG／GG型在自愈组、肝硬化组和肝癌组的OR值（95％CI）分别为1．589（0．735～3．437）、1．351（0．550～3．316）和1．704（0．717～4．052）。各临床特征在不同性别人群中与基因型分布的相关性分析显示，肝癌组中TG／GG型携带者r-GTⅡ阳性的风险显著高于TT型（Х^2=17．534，P=0．001），OR值（95％CI）为14．821（3．227—68．064），特别是在男性中该关联更为密切（Х^2=14．924，P=0．014），OR值（95％CI）为45．000（2．772～730．571）。结论IL-28B基因rs8099917位点与HBV感染后病毒自然清除可能不存在关联性，对慢性肝病进展的影响有待大样本深入分析。Objective To investigate the correlation between IL-28B rs8099917 polymorphism and the outcome of HBV infection. Methods Genotype of rs8099917 （T〉G） in IL-28B locus was determined by TaqMan SNP genotyping from 486 individuals which including 199 chronic HBV carriers （including 100 HBV-induced liver cirrhosis and 99 HBV-related HCC）. 143 people with self- limited infection and 144 healthy people served as controls. Multivariate analysis was used to assess the effect of IL-28B rs8099917 SNP among all the studied groups. Results Distribution of genotype and allele of the rs8099917 locus were in accordance with Hardy-Weinberg equilibrium in different groups or with the total population. The frequencies of the rs8099917 TT, GT, GG genotypes were 89.3%, 10.5% and 0.2%, and the frequency of allele T and G accounted for 94.5% and 5.5%, respectively. In respect of genotype or allele frequency, there was no significant differences found among the groups （P 〉 0.05）. When comparing with the TT genotype, data from the multinomial logistic analysis showed that the ORs and （95%CI） of TG/GG genotypes were 1.589 （0.735-3.437）, 1.351 （0.550- 3.316） and 1.704 （0.717-4.052） , respectively. The genotype frequencies in different groups with different clinical features showed that TG/GG genotypes significantly increased the risk of r-GT Ⅱ （ ＋ ） for individuals with HBV-related HCC （ Х^2= 17.534, P=0.001 ）, with OR as 14.821 （3.227- 68.064）. It was particularly so for males（Х^2= 14.924,P=0.014） ,with 0R（95%CI） as 45.000（2.772- 730.571 ）. Conclusion IL-28B rs8099917 SNP had no correlation with the outcome of HBV infection.

关 键 词：乙型肝炎病毒 自然清除 基因 IL-28B 

分 类 号：R51[医药卫生—内科学]