复方蜥蜴散不同微粒组合剂对CAG模型大鼠细胞凋亡基因Bcl-2与Bax表达影响的实验研究  

Effect of the Compound Lizard Powder on Bcl-2 and Bax Gene Expression in Rats with Chronic Atrophic Gastritis

作　　者：朱西杰[1] 张静霜[1] 李卫强[1] 朱微微[1] 郭利民[1] 苏维霞[1] 徐丽华[1] 

机构地区：[1]宁夏医科大学,宁夏银川750004

出　　处：《时珍国医国药》2011年第1期5-8,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30860360);宁夏医科大学面上项目(No.XM200928)

摘　　要：目的研究复方蜥蜴散不同微粒组合剂对慢性萎缩性胃炎(CAG)细胞凋亡基因Bcl-2和Bax蛋白表达的影响,从分子生物学水平揭示其治疗CAG的机制及其效果。方法将90只SD雄性大鼠随机分为正常组,模型组,复方蜥蜴散大、中、小剂量组及维酶素组,除正常组外,均采取55℃热盐水、2%水杨酸、20mmol/L脱氧胆酸钠3个致萎缩因素配合饥饱失常造成CAG模型。分别运用复方蜥蜴散不同微粒组合剂大、中、小剂量及维酶素治疗,应用免疫组化法检测大鼠胃组织Bcl-2及Bax蛋白表达,光镜观察胃黏膜病理组织学变化。结果通过人工计数法对免疫组化结果分析各组大鼠细胞凋亡基因Bcl-2和Bax蛋白的阳性表达率有显著差异(P<0.05)。通过图像分析系统对免疫组化图像光密度的测算,复方蜥蜴散不同微粒组合剂大、中、小剂量组细胞凋亡基因Bcl-2蛋白的阳性表达率显著低于模型组(P<0.01),复方蜥蜴散不同微粒组合剂大、中剂量组细胞凋亡基因Bcl-2蛋白的阳性表达率显著低于维酶素组(P<0.05)。复方蜥蜴散不同微粒组合剂大、中、小剂量组细胞凋亡基因Bax蛋白的阳性表达率显著高于模型组(P<0.05),复方蜥蜴散不同微粒组合剂大、中剂量组细胞凋亡基因Bax蛋白的阳性表达率显著高于维酶素组(P<0.05)。结论胃黏膜细胞凋亡基因Bcl-2和Bax蛋白表达异常是CAG发生的重要因素之一,复方蜥蜴散不同微粒组合剂对胃黏膜的损害具有保护作用,且能降低Bcl-2蛋白的表达并且提高胃黏膜Bax蛋白表达,部分逆转胃黏膜病理变化,揭示了复方蜥蜴散不同微粒组合剂治疗CAG的作用机制。Objective To investigate the effect of compound lizard powder（TCLP） in different particle combination agents on Bcl-2 protein and Bax protein in rats with chronic atrophic gastritis and its mechanism.Methods Ninety male SD rats were randomly divided into six groups： normal control group,model group,Vitacoenzyme control group,low-dose,middle-dose and high-dose LP.Except normal control group,others were made to model of CAG with 55℃hot water which contained 150 g/L NaCl and 2%Salieylas and 20mmol/L sodium deoxyeholate,matching the abnonmal method of hungy and satisfied diet.The rats were randomly divided into normal control group,model control group,TCLP control group,low-dose TCLP,medium-dose TCLP and high-dose TCLP.All rats were sacrificed,and gastric mucosa were collected to detect Bcl-2 and Bax gene expression and the histological mucosa changes were observed by microscope.Results Positive expresion rates of apoptosis Bcl-2 and Bax gene were significantly different（P 0.05）.Groups of high,middle and low doses of TCLP in different particle combination agents,apoptosis Bcl-2 protein positive gene expression rates significantly lower than in the model group（P 0.01）.Ingroups of large and medium doses,TCLP of apoptosis Bcl-2 protein positive gene expression rates significantly lower than in Vitacoenzyme group（P 0.05）.In groups of large,middle and small doses of TCLP in different particle combination agents,apoptosis Bax protein positive gene expression rates were significantly higher than that of model group（P 0.01）.TCLP in different particle combination agents,large and medium dose groups of apoptosis Bax protein positive gene expression rate was significantly higher than the Vitacoenzyme group（P 0.05）.Conclusion Apoptosis Bax and Bcl-2 gene abnormal protein expression is one of the important factors for CAG occurance.TCLP in different particle combination agents on gastric mucosa damage has protective effect by reducing Bcl-2 gene expression and improving Bax protein expression gastric m

关 键 词：慢性萎缩性胃炎 复方蜥蜴散不同微粒组合剂 Bcl-2 BAX 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]