广西尖吻蝮蛇毒小分子多肽组分对A2780细胞抑制作用的研究  

Anti-tumor Effect of Polypeptide from Guangxi Agkitrodon Acutus Venom on A2780 Cell Line

作　　者：李映新[1] 雷丹青[2] 李剑军[3] 

机构地区：[1]广西医科大学药学院,广西南宁530021 [2]广西医科大学蛇毒研究所,广西南宁530021 [3]广西疾病预防控制中心,广西南宁530028

出　　处：《时珍国医国药》2011年第1期51-53,共3页Lishizhen Medicine and Materia Medica Research

基　　金：广西壮族自治区自然科学基金(No.0575062)

摘　　要：目的探讨广西尖吻蝮蛇毒小分子多肽组分(K组分)对人卵巢癌细胞株A2780的增殖抑制作用以及作用机制。方法采用MTT法检测K组分对癌细胞株的生长抑制情况;黏附实验观察K组分抗细胞黏附作用;采用AO-EB双荧光染色和流式细胞术检测凋亡的发生。结果 K组分对人卵巢癌细胞株A2780的增殖有抑制作用并呈明显的时-效及量-效关系,能够对抗细胞和FN的黏附。AO-EB双荧光染色和流式细胞术都检测到细胞凋亡。结论 K组分对体外培养的人卵巢癌细胞株A2780的增殖有显著的抑制作用,作用机制可能与抗细胞黏附,诱导细胞凋亡有关。Objective To investigate the inhibitory effect of a small peptide extracted from Guangxi Agkitrodon acutus venom（fraction K） on the growth of human ovarian carcimona A2780 cell and mechanism.Methods The inhibition on human cancer cells in vitro was mesured by MTT.Cell adhesion assay was used to observe the effect of fraction K on the adhesion of A2780 cells to fibronectin（FN）.Cells apoptosis induction was detected by acridine orange and ethidium bromide flurescence staining methods（AO-EB） and flow cytometry（FCM） through Annexin V-FITC/PI assay.Results Proliferation of A2780 cells was inhibited significantly by fraction K in dose-effect and time-effect relationship.Incubation with a concentration of 0.80μg/ml for 48h increase the amount of apoptotic A2780 with AO/EB fluorescence staining.The apoptosis was detected by flow cytometry as well.Conclusion Fraction K has a high inhibitory ability against cancer cell growth in vitro,it may be related to cell-adhesion to FN which induces cell apoptosis.

关 键 词：尖吻蝮蛇毒 小肽 抗肿瘤 抗黏附 细胞凋亡 

分 类 号：R284.2[医药卫生—中药学]