制何首乌中四个有效成分含量的高效液相色谱法测定  

HPLC Simultaneous Determination of Four Active Ingredients Content in Radix Polygonum Multiflorum

作　　者：徐向红[1] 平欲晖[1] 谢一辉[1] 

机构地区：[1]江西中医学院,江西南昌330004

出　　处：《时珍国医国药》2011年第1期58-59,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家"十一五"科技支撑计划(No.2006BAI06A07-02)

摘　　要：目的建立高效液相色谱(HPLC)法同时测定制何首乌中二苯乙烯苷、黄豆苷元、大黄素、大黄素甲醚的方法。方法采用高效液相色谱法;色谱柱为Diamonsil C18(5μm,250 mm×4.6 mm);柱温35℃;流速1.2 ml/min;检测波长280nm;流动相为甲醇∶0.5%醋酸溶液(42∶58)。结果被测定峰与其他组分峰完全分离。二苯乙烯苷、黄豆苷元、大黄素甲醚和大黄素的线性范围分别为0.908 4～9.084μg(R2=0.999 8),0.1236～1.236μg(R2=0.999 7),0.6048～6.048μg(R2=0.999 9),0.3648～3.648μg(R2=0.999 2);平均回收率(n=6)分别为100.2%,(RSD=1.9%),98.53%(RSD=2.0%),100.7%(RSD=2.8%)和99.95%(RSD=2.0%)。结论该方法准确、简便、重复性好,可作为制何首乌中二苯乙烯苷、黄豆苷元、大黄素、大黄素甲醚的含量测定方法,并易于推广。Objective To establish HPLC method for the simultaneous derermine of stilbene glycoside,daidzein,emodin and physcion in Radix Polygonum multiflorum.Methods The high-performance liquid chromatography was adopted.The chromatographic column was Diamonsil C18（5μm,250mm×4.6mm）;Column temperature was 35℃,velocity was 1.2ml/min,the detection wavelength 280 nm,and the mobile phase was Methanol： 0.5% acetum（42∶58）.Results The measured peak was completely separated from the other components in the peak.The linear ranges of stilbene glycoside,daidzein,physcion and emodin were 0.908 4～9.084 μg（R2=0.999 8）,0.123 6～1.236μg（R2=0.999 7）,0.604 8～6.048 μg（R2=0.999 9）,0.364 8～3.648μg（R2=0.999 2）,respectively.The average recoveries（n=6） were 100.2%,（RSD=1.9%）,98.53%（RSD=2.0%）,100.7%（RSD=2.8%）和99.95% respectinely（RSD=2.0%）.Conclusion The method is accurate,simple,reproducible and can be used as assay of stilbene glycoside,daidzein,emodin and physcion in Radix Polygoni Multiflori Preparata.

关 键 词：制何首乌 二苯乙烯苷 黄豆苷元 大黄素 大黄素甲醚 多成分同时测定 

分 类 号：R284.2[医药卫生—中药学]