不同性别类型刺五加叶有效成分含量季节积累规律研究  

The Seasonal Accumulating Regularity on Effective Constituent Contents in Leaf Origin from Different Sexual Types of Acanthopanax senticosus

作　　者：孟祥才[1] 颜丙鹏[1,2] 孙晖[1] 王喜军[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]江苏康源药业股份有限公司,江苏连云港222001

出　　处：《时珍国医国药》2011年第1期88-90,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家科技部"十一五"科技支撑计划项目(No.2006BAI06A05-7;2006BAI09B02-07)

摘　　要：目的确定出优质叶片的植物性别类型和最佳采收期。方法采用RP-HPLC色谱法检测金丝桃苷,紫外分光光度法测定不同发育时期总黄酮和总皂苷含量。结果不同性别类型刺五加叶的有效成分含量季节积累规律相似。各生长发育期短花丝类型各主要活性成分的含量显著高于长花丝类型,短花丝类型金丝桃苷、总黄酮和总皂苷年平均含量分别为3.61,20.88,20.42 mg/g,长花丝类型分别2.41,15.95,18.38 mg/g。两种性别类型的各有效成分含量在绿果期后含量较高。在适宜采收期,短花丝类型金丝桃苷、总黄酮和总皂苷年平均含量分别为4.24,27.3,22.7 mg/g,长花丝类型分别2.65,20.5,19.5 mg/g。结论短花丝类型刺五加叶优于长花丝类型,刺五加叶宜在秋季采收。秋季采集不仅可以提高药材的质量和产量,而且还可以减小资源的破坏。Objective To determine the better sexual type and optimal collection period about Acanthopanax senticosus leaves.Methods A validated RP-HPLC method was developed to determine the content of hyperin,and the ultraviolet spectrophotometry method was used to determine total flavonoids and total saponins.Results The season change trends related to active constituent contents of two sexual types were similar.The major active constituents in short filament type were higher than those in long filament type all growth periods,the annual mean contents of hyperin,flavonoids and total saponins were 3.61,20.88 and 20.42 mg/g respectively in the short filament type leaves,2.41,15.95 and 18.38 mg/g in the long filament type leaves.Three kinds of active constituents in A.senticosus leaves were higher after green fruit period.At optimal collective period,the contents of hyperin,flavonoids and total saponins were 4.24,27.3 and 22.7 mg/g in the short filament type leaves,2.65,20.5 and 19.5 mg/g in the long filament type leaves.Conclusion The quality of leaves of short filament type is superior,they should be collected after the green fruit period in autumn.At this time,not only the quality and yield can be improved,but also the destruction of the resource can be reduced.

关 键 词：刺五加 性别 金丝桃苷 总黄酮 总皂苷 

分 类 号：R284.2[医药卫生—中药学]