大孔吸附树脂纯化水黄皮根总黄酮的工艺研究  

Study on Purification of Total Flavonoids in Root of Pongamia pinnataby by Macroporos Resin

作　　者：赵映淑[1] 董志[1] 朱毅[2] 陈国彪[2] 储美娜[1] 

机构地区：[1]重庆医科大学,重庆400016 [2]海南省药品检验所,海南省药物质量研究重点实验室,海南海口570216

出　　处：《时珍国医国药》2011年第1期154-156,共3页Lishizhen Medicine and Materia Medica Research

基　　金：海南省重点科技项目(No.070205)

摘　　要：目的研究大孔吸附树脂纯化水黄皮根总黄酮的最佳工艺。方法选择3种不同大孔吸附树脂对水黄皮根总黄酮进行静态和动态的吸附与解吸;考察上柱原液总黄酮浓度、流速、pH和洗脱剂等对水黄皮根总黄酮优化的影响。结果 D101最适于水黄皮总黄酮的纯化,最佳工艺为:调节水黄皮根提取液总黄酮浓度为0.607 1 mg.ml-1,调pH=3,以2 ml.min-1的流速上样,以相同速率用适量纯化水洗脱(molish反应阴性)后,再用3BV 20%乙醇溶液除杂洗脱,最后用70%乙醇作为洗脱剂进行洗脱。结论经D101大孔吸附树脂分离纯化后,水黄皮根总黄酮浓度可达50%以上。Objective To develop a method for the purification of total flavonoids in root of Pongamia pinnata by macroporous resin with macroporous absorbing resin.Methods The concentration of total flavonoids,flow rate,pH and eluting agent were investigated by three kinds of macroporous resin.Results D101 was the most suitable macroporous resin for the purification technology of total flavonoids in root of Pongamia pinnata.The concentration of loaded solution was 0.607 1mg·ml-1（pH=3） with a flow rate of 2 ml·min-1.The impurities were removed by eluting with purified water（Molish reaction negative）and 20 % achohol（3 BV）,successively.Then the part of eluting with 70 % alcohol was collected.Conclusion The content of total flavonoids in root of Pongamia pinnata was up to 50 % with the optimal purification process.

关 键 词：大孔吸附树脂 水黄皮 总黄酮 纯化 

分 类 号：R284[医药卫生—中药学]