正交实验法优选慢结片的提取工艺  

Optimization of Extraction Process of Manjie Tablets by Orthogonal Design

作　　者：王钢力[1] 张铮[1] 曹杰[2] 张海鸣[2] 杜树山[2] 

机构地区：[1]中国药品生物制品检定所,北京100050 [2]北京师范大学资源学院中药资源保护与利用北京市重点实验室,北京100875

出　　处：《时珍国医国药》2011年第1期191-192,共2页Lishizhen Medicine and Materia Medica Research

基　　金：北京市新医药学科群重点支持项目(No.xk100270569);国家科技支撑计划课题(No.2007BAI48B10)

摘　　要：目的优选慢结片的最佳提取工艺。方法以干膏率、盐酸小檗碱提取量为评价指标,选择加乙醇浓度、溶剂量、提取时间、提取次数为考察因素,利用L9(34)正交实验确定慢结片的提取工艺。结果最佳提取工艺为70%乙醇提取3次,10倍量提取2h、8倍量提取1h、8倍量提取1h。结论优选出的提取工艺简单、可行,适应药厂生产需要,可作为该制剂合理开发的依据。Objective To make the optimum process conditions of extraction for Manjie tablets.Methods Applying optimum extraction of Manjie tablets coptidis with berberine hydrochloride and dry-paste as marker was investigated by orthogonal design.Results The optimal process conditions were as follows： the concentration of ethanol 70%,reflux-extraction for 2hrs with 10 times volume,1hr with 8 times,1hr with 8 times.Conclusion This method is simple and applied rightly in mass production and it can provide the references for Manjie tablets for its further investigation.

关 键 词：慢结片 提取工艺 正交实验 

分 类 号：R284.2[医药卫生—中药学]