硒化壳聚糖对NB4细胞的生长抑制作用与Ras信号通路的关系(英文)  

Correlation ship between Inhibitory Effect of Selenium Chiston on NB4 Cells and Ras Signal Transduction Pathway

作　　者：邓守恒[1] 邰云燕[1] 杨敬宁[2] 李芳[1] 姜立东[1] 陈萍[1] 

机构地区：[1]郧阳医学院附属人民医院肿瘤中心,湖北十堰442000 [2]郧阳医学院免疫学教研室,湖北十堰442000

出　　处：《时珍国医国药》2011年第1期240-243,共4页Lishizhen Medicine and Materia Medica Research

基　　金：project supported by the Education Commission of Hubei province of China (grant No.B200624004)~~

摘　　要：目的研究硒化壳聚糖(Sc)对急性早幼粒细胞白血病(APL)细胞株NB4增殖的影响,探讨这种影响与PML-RARα融合蛋白及其激活的Ras信号途径的关系。方法 NB4为表达PML-RARα融合蛋白阳性的细胞株,K562为表达PML-RARα融合蛋白阴性的细胞株,应用MTT法检测Sc对两种细胞株增殖的影响,应用流式细胞术和Western blot的方法检测两种细胞株相关蛋白含量的变化。结果 Sc对两种细胞株均可产生剂量和时间依赖性的抑制作用,相比之下,对K562细胞的抑制作用明显较弱(P<0.05)。Sc处理后,NB4细胞内PML-RARα融合蛋白和c-Jun信号蛋白含量明显减少,而K562细胞内c-Jun信号蛋白含量则轻微减少。结论 Sc可特异性地减少PML-RARα融合蛋白的含量从而下调其激活的Ras信号途径,最终抑制NB4细胞增殖。Objective To investigate the effects of Selenium chiston（Sc） on proliferation of NB4 cells and the relationship between this effect and Ras signal transduction pathway activated by PML-RARα fusion proteins.Methods NB4 cell line was used as a PML-RARα fusion proteins-positive cell system and K562 cell line as a PML-RARα fusion proteins-negative control.MTT was used to determine the proliferative effects of drugs on NB4 and K562 cells.Western blot and flow cytometry were used to examine the abundance of signal protein molecules expressed in tumor cells.Results An exposure of NB4 cells or K562 cells to Sc produced both concentration and time-dependent increase in the anti-proliferative rate.Moreover,NB4 cells had more sensitivity to Sc than K562 cells（P0.05）.The abundance of PML-RARα fusion proteins as well as c-JUN proteins were strongly down-regulated in Sc-treated PML-RARα fusion proteins positive NB4 cells while c-JUN proteins were only slightly down-regulated in PML-RARα fusion proteins negative K562 cells.Conclusion Selenium chiston inhibited the proliferation of NB4 cells and the inhibitory effect was correlated with down-regulation of the abundance of PML-RARα fusion proteins,which may ultimately lead to retard the Ras Signal transduction pathway.

关 键 词：硒化壳聚糖 NB4细胞 C-JUN PML-RARΑ融合蛋白 

分 类 号：R284.2[医药卫生—中药学]