荜茇醇提物对胰岛素抵抗综合征模型大鼠免疫功能影响的实验研究  

Experimental Study on the Effect of Piper longum L.on Immune Function in Rats with Insulin Resistance Syndrome

作　　者：宋娜丽[1] 包.照日格图[2] 万春平[3] 唐志国[2] 方路[1] 马克坚[1] 

机构地区：[1]云南省中医中药研究院,云南昆明650223 [2]云南中医学院国家中医药管理局中药药理(免疫)实验室,云南昆明650500 [3]上海中医药大学病毒与免疫实验室,上海201203

出　　处：《时珍国医国药》2011年第5期1081-1083,共3页Lishizhen Medicine and Materia Medica Research

基　　金：云南省自然科学基金(No.2005C0014R)

摘　　要：目的观察荜茇乙醇提取物(Ethanol Extract of Piper LongumL.,EEPL)对胰岛素抵抗综合征(IRS)模型大鼠免疫功能的影响。方法采用高脂高糖高盐膳食造模,高脂饲料造模4周后,各组给予相应药物,给药8周后处死大鼠,于无菌条件下取大鼠脾淋巴细胞、自然杀伤细胞(NK)和腹腔巨噬细胞(Mφ),采用中性红法检测Mφ吞噬功能,MTT法检测T、B淋巴细胞增殖活性和NK细胞的自然杀伤力。结果与模型对照组比较,EEPL高、中剂量组T、B淋巴细胞增殖率明显升高(P<0.05或P<0.01),EEPL各剂量组NK细胞杀伤率和Mφ吞噬能力明显提高,差异有统计学意义(P<0.05或P<0.01)。结论 EEPL具有改善IRS模型大鼠免疫功能的作用。Objective To observe the effect of ethanol extract of Piper longum L.（EEPL） on immune function in rats with insulin resistance syndrome（IRS）.Methods The rat model was induced by feeding with high-fat,high-glucose and high-salt diet.in the 4th week,each group was given related medicine,rats were killed after treating with medicine for 8 weeks,the phagocytosis of Mφ（method of colorimetric） in rat abdominal cavity,the proliferation activity of splenic T and B-lymphocyte and the cytotoxic activity of splenic natural killer（NK） cells（method of MTT） were detected under aseptic condition.Results Compared with model group,the proliferation activity of splenic T and B-lymphocytes was increased in EEPL high and medium groups obviously（P0.05 or P0.01）,the cytotoxic activity of splenic NK cells and the phagocytosis of Mφ in each EEPL group were enhanced,and the difference was statistically significant（P0.05 or P0.01）.Conclusion EEPL can improve immune regulation in rats with IRS.

关 键 词：荜茇醇提物 胰岛素抵抗综合征 免疫功能 

分 类 号：R962[医药卫生—药理学] R291.2[医药卫生—药学]