三黄方抗内毒素活性的谱效相关性研究  

Spectrum efficiency Relationship Research on Anti-endotoxin Activity of San-Huang Formula

作　　者：邓翀[1] 张艺[2] 孟宪丽[2] 王张[2] 范刚[2] 

机构地区：[1]陕西中医学院,陕西咸阳712046 [2]成都中医药大学,四川成都610075

出　　处：《时珍国医国药》2011年第3期525-527,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30772764;30371756)

摘　　要：目的考察三黄方制剂化学指纹图谱与体外抗内毒素活性的相关性,为提高三黄方制剂质量标准提供依据。方法采用HPLC建立12个厂家三黄方制剂的化学指纹图谱,并采用动态浊度法测定其不同稀释度的抗内毒素活性;运用多元线性回归、双变量相关分析、主成分分析统计分析指纹图谱和体外抗内毒素活性的相关性。结果小檗碱、黄芩苷、大黄酸、大黄素、大黄酚、大黄素甲醚等化学成分和体外抗内毒素活性相关。结论可以以黄芩苷、大黄素、大黄酚、大黄酸、大黄素甲醚为指标,对三黄方质量进行控制。Objective To study the relationship between chemical fingerprints and San-Huang Formula Preparations activity,so to provide the basis for quality standards of San-Huang Formula Preparations. Methods Chemical fingerprint of the preparation from 12 manufacturers was built by HPLC,and the relationship between firgerprint and anti-endotoxin activity in vitro was analyzed by multiple linear regression,bivariate correlation analysis,principal component analysis. Results Chemical compositions such as berberine,baicalin,rhein,emodin,chrysophanol,rhubarb were related with in vitro anti-endotoxin activity. Conclusion Baicalin,emodin,chrysophanol,rhein,rhubarb can be used as indicator of the quality for San-Huang Formula Preparations.

关 键 词：三黄方 指纹图谱 体外抗内毒素活性 谱效学 

分 类 号：R289[医药卫生—方剂学]