小剂量硒化壳聚糖与全反式维甲酸协同诱导NB4细胞分化和凋亡  

Apoptosis and Differentiation Induced by Low Dose Selenium Chitosan Combined with Low Dose All-trans Retinoic Acid in NB4 Cells

作　　者：邓守恒[1] 李林均[1] 蔡晓军[1] 曹凤军[1] 李芳[1] 陈萍[1] 

机构地区：[1]郧阳医学院附属人民医院肿瘤中心,湖北十堰442000

出　　处：《时珍国医国药》2011年第3期593-595,共3页Lishizhen Medicine and Materia Medica Research

基　　金：湖北省教育厅科研基金(No.B200624004);郧阳医学院科研基金资助项目(No.20081207)

摘　　要：目的研究小剂量硒化壳聚糖(25 mg/L)与全反式维甲酸(ATRA 0.1μmol/L)联合应用对人急性早幼粒细胞白血病细胞株NB4分化及凋亡的影响。方法采用DNA片段化凝胶电泳法检测细胞凋亡;NBT还原法检测细胞分化;流式细胞法检测分化抗原CD11b。结果 25 mg/L硒化壳聚糖没有诱导NB4细胞分化和凋亡的能力,但与0.1μmol/L ATRA联合使用同单独使用0.1μmol/L ATRA相比,CD11b表达阳性率进一步增加,NBT还原能力进一步增强,两药合用DNA电泳呈现出典型的梯带。结论小剂量硒化壳聚糖与ATRA联合使用可诱导NB4细胞发生凋亡及分化。Objective To study the effects of low dose selenium chitosan combined with all-trans retinoic acid on apoptosis and differentiation of human acute promyelocytic Leukemia NB4 cells. Methods Apoptosis was detected by DNA fragmentation by agarose gel electrophoresis analysis.Cell differentiation was measured by flow cytometry of CD11b expression and NBT reduction assay. Results 25 mg/L selenium chitosan alone could not induce apoptosis of NB4 cells.However,combination of 25mg/Lselenium chitosan and 0.1 μmol/L ATRA for 48 hours could induce significant apoptosis characterized by DNA ladder.Selenium chitosan at concentration of 25 mg/L was not able to induce differentiation of NB4 cells,but when combined with 0.1 μmol/L ATRA,CD11b expression and NBT reduction were increased as compared with that of 0.1 μmol/L ATRA alone. Conclusion Low dose selenium chitosan can enhance the effects of low dose ATRA in inducing apoptosis and differentiation of NB4 cells.

关 键 词：硒化壳聚糖 全反式维甲酸 凋亡 分化 NB4细胞 

分 类 号：Q254[生物学—细胞生物学]