蒙药复方森登-4中总萜含量的紫外可见分光光度法测定  

Determination of Total Terpene Content in Mongolian Medicine Compound Senden-4 by UV-Vis Spectrophotometry

作　　者：马强[1,2] 武娜[1] 董玉[1] 贾鑫[1] 

机构地区：[1]内蒙古医学院.药学院,内蒙古呼和浩特010059 [2]内蒙古科技厅,内蒙古呼和浩特010010

出　　处：《时珍国医国药》2011年第4期819-820,共2页Lishizhen Medicine and Materia Medica Research

基　　金：内蒙古自治区自然科学基金(No.2009Zd18);内蒙古自治区重大科技项目;内蒙古科技厅社会发展领域科技计划项目

摘　　要：目的建立测定森登-4中总萜的含量的方法。方法采用紫外可见分光光度法测定,检测波长为446 nm。结果栀子苷在浓度0.006 08~0.097 28 mg范围内呈良好线性关系。回归方程Y=8.276X＋0.008 7（r=0.999 8）。线性关系良好,加样回收率为98.95%,RSD为1.52%。结论该方法简便、灵敏、准确,可用于森登-4中总萜的含量测定。Objective To develop a method for the decermination of total terpene content in senden-4.Methods UV-visible spectrophotometry was adopted and detection wavelength was 446nm.Results There had a good linear relationship whthin the range of 0.006 08~0.097 28 mg for gemiposide.The regression equation was Y = 8.276X ＋0.008 7（r = 0.999 8）,the recovery was 98.95%,and RSD was 1.52%.Conclusion This method is simple,sensitive,accurate,and can be used for determination of total terpene in senden-4.

关 键 词：森登-4 总萜 紫外可见分光光度法 

分 类 号：R284.2[医药卫生—中药学]