破布叶枝和叶中总三萜酸的含量测定  

Determination of Triterpenoidic Acid Content in Stems and Leaves of Microcos paniculata L.

作　　者：鲍长余[1] 刘冰晶[1] 毕和平[1,2] 陈光英[1,2] 范超君[1] 辜燕飞[1] 

机构地区：[1]海南师范大学化学与化工学院,海南海口571158 [2]海南省热带药用植物化学重点实验室,海南海口571158

出　　处：《时珍国医国药》2011年第4期845-846,共2页Lishizhen Medicine and Materia Medica Research

基　　金：海南省自然科学基金(No.20804);海南省热带药用植物重点实验室资助项目;海南师范大学大学生创新实验项目

摘　　要：目的建立了测定破布叶枝和叶中总三萜酸含量的方法。方法以熊果酸为对照品,以质量分数5%香草醛-冰醋酸和高氯酸溶液系统为显色剂,采用紫外分光光度法在546 nm波长处测定样品的吸光度。结果熊果酸对照品在0.20~1.60μg.ml-1范围内呈良好线性关系,回归方程为Y=0.154 8C＋0.028 6,r=0.999 3。破布叶枝和叶中的总三萜酸含量分别为1.09%和2.77%。结论该法操作简便,结果可靠,重复性好,可用作破布叶中总三萜酸的含量测定。Objective To establish a method for the determination of the total triterpenoidic acid in stems and leaves of M.paniculata L.Methods With ursolic acid as the reference,5% vanillin-acetic acid and perchloric acid as color developer,the total triterpenoidic acid in stems and leaves of M.Paniculata L.were determined by ultraviolet spectrophotometric method at 546 nm wavelength.Results Sample size showed a good linear relationship within the range of 0.20～1.60 μg ·m L-1,and the curve was Y= 0.154 8 C ＋0.028 6,r = 0.999 3.The content triterpenoidic acid in stems and leaves was 1.09 % and 2.77 %,respectively.Conclusion The spectrophotometric method is simple,reproducible and suitable for the determination of total triterpenoidic acid.

关 键 词：破布叶 三萜酸 紫外分光光度法 

分 类 号：R284.2[医药卫生—中药学]