川芎嗪对MPTP诱导的多巴胺能神经元毒性的影响  

Effect of TMP on Dopaminergic Neuron Injury Induced by MPTP in vivo and vitro

作　　者：杜静[1] 单璐琛[1] 张高小[1] 王玉强[1] 

机构地区：[1]暨南大学药学院新药研究所,广东广州510632

出　　处：《时珍国医国药》2011年第7期1564-1565,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30973618);国家重大新药创制(No.2009ZX09103-031)

摘　　要：目的观察川芎嗪体外、体内对MPTP导致的多巴胺能(dopaminergic,DA)神经元毒性的影响。方法体外实验,提取孕14 d的Sprague-dawley大鼠胎鼠黑质区多巴胺神经元,MPP+诱导其损伤,运用免疫荧光法测定黑质酪氨酸羟化酶(tyrosine hydroxylase,TH)阳性神经元数量及状态变化;体内实验,以C57BL/6J小鼠为研究对象,采用腹腔注射MPTP方法制作小鼠慢性帕金森(Parkinson's disease,PD)模型,运用免疫组化法测定TH阳性神经元数量变化。结果体外实验:TMP组多巴胺神经元形态数目均有所改善,其中500μmol/L组与模型组相比具显著性差异。体内实验:与模型组相比,注射TMP后,多巴胺神经元密度有所增加。结论 TMP能不同程度地改善MPTP诱导的多巴胺神经元的损伤。ObjectiveTo investigate the effect of tetramethylpyrazine（TMP） on dopaminergic neuron injury induced by N-methyl-4-phenyl-1,2,3,6-tetrahydro-pyridine（MPTP） in vitro and in vivo.MethodsIn vitro： Rat mesencephalic neurons were cultured for 5 days,followed by addition of MPP＋.The number and morphology of positive TH neurons were observed using an inverted fluorescence microscope.In vivo： Mice were administered MPTP（25 mg/kg,i.p,per day） for 5 days to establish the PD animal model.The number of positive TH cells in the SNpc was observed by immunohistochemistry.ResultsIn vitro： The number of dopaminergic neurons and the neurite length in the TMP group were increased compared to that of the MPP＋ group.In vivo,TMP treatment increased the density of dopaminergic neuron injury induced by MPTP.ConclusionTMP can protect dopaminergic neuron injured by MPP＋ and is beneficial in MPTP-induced PD animal model.

关 键 词：川芎嗪 MPTP MPP+ 多巴胺神经元 

分 类 号：R245.3[医药卫生—针灸推拿学]