清络通痹颗粒对胶原性关节炎大鼠以及血管内皮细胞增殖的影响  

Effects of Qingluo Tongbi Granula on Collagen-induced Arthritis Rats and Proliferation of ECV304

作　　者：梁晓雯[1] 许立[1] 周静[1] 王昀[1] 程旸[1] 柳璋璞[1] 周玲玲[1] 

机构地区：[1]南京中医药大学,江苏南京210029

出　　处：《时珍国医国药》2011年第7期1666-1668,共3页Lishizhen Medicine and Materia Medica Research

基　　金：江苏省高校自然科学基金(No.08KJB360006)

摘　　要：目的观察清络通痹颗粒(QLT)对胶原性关节炎大鼠血液流变学,外周血IL-1表达的影响以及对血管内皮细胞增殖的影响。方法制备胶原性关节炎大鼠模型(CIA),检测QLT对胶原性大鼠血液流变学和外周血IL-1的影响,MTT比色法检测QLT含药血清对血管内皮细胞增殖的影响。结果 QLT 7.2,14.4g/kg剂量组全血粘度及血浆黏度、外周血IL-1表达水平与模型组相比有明显降低,并且可显著抑制ECV304细胞的增殖。结论 QLT3个剂量组可不同程度降低全血黏度和血浆黏度,从而达到活血化淤的作用;抑制血管内皮细胞的增殖,从而达到抑制血管增生和血管生成,这可能是清络通痹颗粒治疗RA的重要机制。ObjectiveTo explore the effects of Qingluo Tongbi Granula on hemorheology,IL-1 expression in serum of collage-induced arthritis（CLA） rats and proliferation of ECV304.MethodsThe CIA rats model was established and hemorheology and IL-1 expressin in peripheral blood of CIA rats were detected.MTT assay was used to examine the effect of QLT-containing serum on the proliferation of ECV304.ResultsThe levels of whole blood viscosity and plasma viscosity,IL-1 expression in peripheral blood by the dose of QLT 7.2,14.4 g / kg were significantly lower than those of model group.Besides,the proliferation of ECV304 was inhibited by QLT-containing serum.ConclusionThe three doses of OLT could decrease the blood viscosity and plasma viscosity at different levels,inhibit proliferation of ECV304,whicth may be important mechanism for Qingluo Tongbi Granula to treat RA.

关 键 词：清络通痹颗粒 CIA大鼠 血液流变学 IL-1 ECV304细胞 

分 类 号：R285.5[医药卫生—中药学]