3种五味子药材HPTLC鉴别与HPLC含量测定  

Identification and Determination of Three Kinds of Schisandra by HPTLC and HPLC

作　　者：李立顺[1] 时维静[1] 王贵阳[1] 

机构地区：[1]安徽科技学院,安徽凤阳233100

出　　处：《时珍国医国药》2011年第7期1698-1699,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家"十一五"科技支撑计划重点项目(No.2008BADB4B06);安徽科技学院项目(No.X201080)

摘　　要：目的建立五味子HPTLC定性鉴别和HPLC含量测定方法。方法以五味子甲素、五味子乙素、五味子醇甲、五味子酯甲为对照品,采用HPTLC法带状点样于GF254板上,以石油醚(30～60℃)-甲酸乙酯-甲酸(15∶5∶1)为展开剂,上行展开8 cm,置于紫外灯254nm下观察,比较不同来源的五味子活性成分的差异。以五味子醇甲为对照品,采用HPLC法,ZORBAX SB-C18(4.6 mm×250 mm,5μm),流动相:甲醇-水(65∶35);流速:1.0 ml.min-1;柱温:室温;检测波长:254 nm,比较不同来源的五味子醇甲的含量。结果 HPTLC法能够准确快速鉴别不同来源的五味子;3种药材中的五味子醇甲含量有差异。结论该方法简便、准确、重现性好,可从定性、定量两方面评价五味子药材质量。ObjectiveTo establish HPLC and HPTLC methods for identification and determination of Schisandra.MethodsChromatographic conditions were as follows： deoxyschizandrin,γ-Schisandrin,Schisandrin,Schisantherin as control samples;band application on GF254 plate;developer solvent system： ligarine（30～60℃）-ethyl formate-methanoic acid（15∶5∶1）;distance from lower edge of plate 8 mm and observing the fluorescent chromatogram in a UV cabinet at 254 nm.The sample solution was analyzed by HPLC.The assay was performed on a Zorbax SB-C18 column（4.6 mm×250 mm,5 μm）.The mobile phase was： methanol：H2O=（65∶35）,the flow rate was 1.0 ml·min-1,the detection wavelength was 254nm and column temperature was room temperature.ResultsHPTLC method could quickly and accurately identify different sources of Schisandra and three kinds of herbs were different in the content of schisandrin.ConclusionThe methods are simple,accurate and reproducible,which can evaluate the quality of Schisandra qualitatively and quantitatively.

关 键 词：五味子 HPTLC HPLC 

分 类 号：R284.2[医药卫生—中药学]