云南松松塔木质素-糖复合物中的糖含量测定  

Determination of Carbohydrate Content in Lignin-Carbohydrate Complex from Pine Cone of Pinus yunnanensis Franch

作　　者：周萍[1] 林樊[1] 刘洁[1] 吕永俊[1] 李好枝[1] 刘光明[1] 

机构地区：[1]大理学院.药学院,云南大理671000

出　　处：《时珍国医国药》2011年第8期1820-1821,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30860365)

摘　　要：目的测定云南松松塔木质素-糖复合物中的糖含量。方法采用热水、碱水依次提取,乙醇分级沉淀的方法提取出木质素-糖复合物,采用苯酚-硫酸法在波长485.4 nm处进行了糖含量的测定。结果 求得标准曲线的回归方程为Y=0.055 2X+0.014 1(r=0.999 4)。平均回收率为100.4%(RSD=0.40%)。4种样品C,D,E,F的糖含量分别为17.08%,17.51%,28.38%,23.67%。结论 实验方法简便易行,准确度高,重复性好。Objective To determine the content of carbohydrate in lignin-carbohydrate complex from pine cone of P.yunnanensis Fr.Methods Lignin-carbohydrate complex was extracted from pine cone of P.yunnanensis Fr.with the method of hot water followed by alkaline solvent extraction and fractional precipitation with ethanol.The content of carbohydrate fraction in lignin-carbohydrate complex was measured by phenol-sulfuric acid method at 485.4nm.Results The regression equation was Y=0.055 2X＋0.014 1（r=0.999 4）.The average recovery was 100.4 %（RSD=0.40%）.The carbohydrate contents in sample C,D,E,F were 17.08%,17.51%,28.38% and 23.67%,respectively.Conclusion The method is simple,accurate and reproducible.

关 键 词：云南松松塔 木质素-糖复合物 苯酚-硫酸法 

分 类 号：R284.2[医药卫生—中药学]