地鳖纤溶蛋白对人脐静脉内皮细胞、肺癌、乳腺癌细胞VEGF和bFGF表达的影响  

Effect of Eupolyphaga Fibrinolytic Protein on the Expression of VEGF and bFGF in HUVEC,A549,MCF-7 Cells

作　　者：曹付春[1] 韩雅莉[1] 陈冰[1] 黄明星[1] 丁鸿[1] 余磊[1] 叶韵[1] 

机构地区：[1]广东工业大学轻工化工学院,广东广州510006

出　　处：《时珍国医国药》2011年第8期1827-1829,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30772739);广东工业大学轻工化工学院211工程培育项目(No.20091015)

摘　　要：目的研究地鳖纤溶蛋白(EFP)对人脐静脉内皮细胞(HUVEC)和人肺癌(A549)、乳腺癌(MCF-7)细胞分泌血管内皮细胞生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF)的影响。方法采用含药血清体外培养人脐静脉内皮细胞、肺癌、乳腺癌细胞,通过MTT法和双抗夹心酶联免疫吸附分析法(ELISA)观察地鳖纤溶蛋白对细胞的体外增殖和分泌VEGF和bFGF的影响。结果 EFP含药血清对人脐静脉内皮细胞、肺癌、乳腺癌细胞有显著抑制作用,ELISA法检测结果表明EFP含药血清对人脐静脉内皮细胞、肺癌、乳腺癌细胞的VEGF和bFGF表达均有抑制作用。结论 EFP可抑制肿瘤细胞VEGF和bFGF表达。Objective To study the effect of Eupolyphaga fibrinolyric protein（EFP） on the expression of vascular endothelial growth factor（VEGF） and basic fibroblast growth factor（bFGF） in human umbilical vein endothelial cell line（HUVEC）,human lung cancer cell line A549,human breast cancer cell line MCF-7.Methods HUVEC,A549,MCF-7 cells were cultured with EFP drug serum in vitro,MTT colorimetric assay was used to observe the inhibition on the proliferation of cells and double antigen sandwich enzyme-linked immunosorbent assay（ELISA） was adopted to study the effect of EFP on the expression of VEGF and bFGF by determining the contents in cell culture.Results EFP drug serum had obvious inhibition on the proliferation of HUVEC,A549,MCF-7 cells,and had expression levels of VEGF and bFGF in HUVEC,A549 and MCF-7 cells.Conclusion EFP can inbibit the expression of VEGF and bFGF.

关 键 词：地鳖虫纤溶活性蛋白 HUVEC细胞 A549细胞 MCF-7细胞 VEGF BFGF 

分 类 号：O629[理学—有机化学]