苦参药材中总碱含量的溴甲酚绿比色法测定  

Determination of Total Alkaloids in Radix Sophora Lavescens by Acid Dye Colorimetry

作　　者：王淼[1] 韩丽[1] 谢兴亮[2] 赵强强[1] 熊永爱[1] 陈璐[1] 杨明[1,3] 

机构地区：[1]成都中医药大学中药材标准化教育部重点实验室,四川成都611137 [2]成都医学院,四川成都610083 [3]江西中医学院现代中药制剂教育部重点实验室,江西南昌330004

出　　处：《时珍国医国药》2011年第8期1877-1878,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家科技重大新药创制专项(No.2009ZX09103-307)

摘　　要：目的建立苦参药材中生物碱类成分的含量测定方法。方法取苦参的酸水提取液,调节pH值至7.00左右,加入pH3.6的柠檬酸-磷酸氢二钠缓冲液,溴甲酚绿试液,振摇后用氯仿萃取,静置后分取氯仿层,于417.0 nm处测定吸光度。结果 苦参碱检测浓度在0.029 6～0.103 6 mg.m l-1范围内与吸光度线性关系良好,平均加样回收率为100.61%(RSD=2.00%),并对实际药材进行了含量测定。结论 该方法操作简便、准确,可用于苦参药材的质量控制。Objective To establish a method for determination of total alkaloids in radix Sophora lavescens by acid dye colorimetry.Methods Total alkaloids of radix Sophora lavescens were extracted by acidified water,adjusted to neutral by sodium hydroxide solution,dissolved with bufer solution,and mixed with 0.14% of bromocresol green solution and chloroform.The mixture was shaken,and the absorbability of chloroform layer was detected at the wavelength of 417.0 nm.Results The detection concentration of matrine showed good linearity with its absorbability within the range of 0.029 6~0.103 6 mg·ml-1,the average recovery was 100.61%（RSD=2.00%）.Three radix Sophora lavescens from different source were determined with this method.Conclusion The established method is simple,accurate,and can be used for quality control of Radix Sophora flavescentis.

关 键 词：溴甲酚绿 酸性染料比色法 氧化苦参碱 含量测定 

分 类 号：R284.2[医药卫生—中药学]