不同电针频率对肥胖大鼠下丘脑瘦素受体和胰岛素受体基因表达的影响  

Effect of Different Frequency Electroacupuncture on Expression of LR and IR in Hypothalamus of Obese Rats

作　　者：龚美蓉[1] 王耘[1] 姜军作[2] 徐斌[1] 

机构地区：[1]南京中医药大学针药结合实验室,江苏南京210029 [2]大连大学附属新华医院,辽宁大连116021

出　　处：《时珍国医国药》2011年第8期2031-2033,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30873307);国家自然科学基金(No.30901923);江苏省教育厅科研项目(No.07KJA36028);教育部"高等学校博士学科点专项科研基金"(No.20093237110009)

摘　　要：目的通过比较不同电针频率刺激对肥胖大鼠体质量,脂肪量,下丘脑瘦素受体和胰岛素受体基因表达的影响,探讨不同电针频率的效应差异。方法随机挑选10只普通饲料喂养为正常组;另一组80只高脂致肥饲料喂养,将造模成功的肥胖大鼠40只随机分为肥胖模型组和针刺组(2 Hz,2/15 Hz,15Hz),每组各10只。采用实时定量PCR技术测定下丘脑瘦素受体(LR)和胰岛素受体(IR)基因表达水平。结果 不同频率电针治疗均可降低肥胖大鼠的体重和脂肪量,下丘脑组织中LR与IR较模型组均上升。其中高频组电针组优于低频电针组,2/15 Hz频率疗效优于高频与低频电针组。结论 不同电针频率刺激肥胖大鼠,可能通过减少脂肪量和提高下丘脑瘦素受体与胰岛素受体,达到减肥效应,且2/15 Hz频率疗效优于高频与低频电针组。Objective To observe the effect of different frequency of electroacupuncture（EA） on body weight,fat,and the expression of LR and IR in hypothalamus of obese rats.Methods 10 rats fed with ordinary forage were used as normal control group,and other 80 rats fed with high fat forage.Successfully established experimental model rats（n=40） were randomly divided into four groups： one group without any treatment as model control group（control group,n=10）,and the other were treated with electroacupuncture（2Hz,2/15Hz,15Hz,n=10）.LR and IR mRNA were detected with SYBR Green quantitative real-time PCR.Results The body weight,fat were lower and the expression of LR and IR in hypothalamus were higher in different frequency EA groups.The effect of high-frequency EA was superior to that of low-frequency EA,and the effect of 2 / 15 Hz frequency was the best.Conclusion Different frequency EA can effectively lose weight through fat reduction and LR and IR elevation in hypothalamus.The effect of 2 / 15 Hz frequency is better than that of both high-frequency and low-frequency.

关 键 词：肥胖大鼠 电针频率 瘦素受体 胰岛素受体 

分 类 号：R284.2[医药卫生—中药学]