艾灸对类风湿关节炎模型大鼠跖关节中细胞因子的影响  

Effect of moxibustion on the metatarsal joint cytokine in rheumatoid arthritis rats

作　　者：罗磊[1,2] 胡玲[1] 宋小鸽[1] 唐照亮[1] 何璐[1] 蔡荣林[1] 郝峰[1] 

机构地区：[1]安徽中医学院,合肥230038 [2]湖北中医药大学,武汉博士研究生430000

出　　处：《环球中医药》2011年第6期416-419,共4页Global Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)(2009CB522905)

摘　　要：目的观察艾灸"肾俞"、"足三里"对类风湿关节炎(Rheumatoid arthritis,RA)模型大鼠跖关节中细胞因子的影响,为艾灸的局部抗炎免疫作用机制提供依据。方法采用风、寒、湿环境因素结合弗氏完全佐剂的模型复制方法;将120只Wistar大鼠随机分为正常组、模型组、药物组、针刺组、艾灸组、CO2激光组六组,每组20只;分别以艾灸、针刺、药物、10.6μmCO2激光照射为主要治疗手段,运用免疫组化染色,观察艾灸"肾俞"、"足三里"对模型大鼠局部跖关节中白细胞介素-1(IL-1)、白细胞介素-2(IL-2)、肿瘤坏死因子-α(TNF-α)的影响。结果艾灸具有调节局部免疫紊乱的作用,使局部跖关节中IL-1和TNF-α水平下调,IL-2水平上调。结论艾灸对局部关节组织中细胞因子的调节是艾灸发挥抗炎免疫作用的另一可能有效机制。Objective To observe the influence on the metatarsal joint cytokine in rat models with rheumatoid arthritis after moxibustion on Shenshu（BL 23） and Zusanli（ST 36） points,and to provide the new evidence for the local anti-inflammatory immune effect of moxibustion.Methods RA model in rats were duplicated with Wind,cold,wet environmental factors combined with Freund＇s complete adjuvant.The aminals were divided into normal group,moxibustion group,acupuncture group,drug group and CO2 laser group,and were treated with corresponding methods,which were with no treatment,moxibustion,acupuncture,drugs and 10.6 μmCO2 laser irradiation.IL-1β,IL-2 and TNF-αwere tested by immunohistochemical method to discuss the effect of moxibustion Shenshu（BL 23） and Zusanli（ST 36） on the local joint cytokine.Results Moxibustion could increase IL-1β and TNF-α and decreased IL-2 in metatarsal joints of rheumatoid arthritis model rats,and could adjust the function of local immune disorders.Conclusion The anti-inflammatory immune function of moxibustion could be achieved by adjusting the cytokine in local joint tissue.

关 键 词：艾灸 类风湿关节炎 跖关节 细胞因子 

分 类 号：R285.5[医药卫生—中药学]