野猪Toll样受体基因的结构和功能鉴定  

Structural and functional identification of Toll-like receptor genes in wild boar

作　　者：杨秀芹[1] 李海涛[1] 徐扬[1] 翟春媛[1] 马建章[2] 刘娣 

机构地区：[1]东北农业大学动物科技学院,哈尔滨150030 [2]东北林业大学野生动物资源学院,哈尔滨150040

出　　处：《兽类学报》2011年第4期428-432,共5页Acta Theriologica Sinica

基　　金：国家自然科学基金资助项目(31972007);中国博士后科学基金特别资助项目(200902426);哈尔滨市科技创新人才研究专项基金(2008RFQXN018);黑龙江省自然科学基金项目(200927)

摘　　要：Toll样受体（Toll-like receptors,TLRs）是介导天然免疫和获得性免疫的病原模式识别受体（Pattern recognition receptor,PRRs）,能识别表达在病原微生物上高度保守的病原相关分子模式（Pathogen associated molecular patterns,PAMPs）,并通过一定的信号转导途径引起核内相关基因的表达,启动和调节机体的免疫反应。Toll-like receptors（TLRs）,which play an important role in the immune responses,are candidate genes for resistance to a large number of diseases in animals.In order to provide more useful information for analyzing the structure and function of TLRs,this study was designed to clone TLR4/5 genes in Wild boar and to characterize their tissue expression patterns using RT-PCR.The complete coding sequences of Wild boar TLR4/5 genes were obtained,and each predicted polypeptide contained important structural domains including signal peptide,LRRs and TIR.Wild boar TLR4/5 genes were expressed ubiquitously in all tissues tested.The results will provide molecular biological evidence further revealing the structure and function of TLR4/5.

关 键 词：野猪 TLR4/5 克隆 组织表达 

分 类 号：Q751[生物学—分子生物学]