大肠杆菌中整合状态的F′质粒复制起点的克隆和分析  

Cloning and Study on the Replication Origin of the Integrated F Plasmid in Escherichia colt

作　　者：毛裕民[1] 盛祖嘉[1] 

机构地区：[1]复旦大学遗传学研究所

出　　处：《Acta Genetica Sinica》1990年第2期148-153,共6页

基　　金：国家自然科学基金(3870279)

摘　　要：用标记获救法克隆了整合状态的F′质粒的复制起点,证明了由这一复制起点构成的mini-F质粒在不亲和性和对吖啶橙的敏感性方面和自主状态的F′质粒都没有不同。对这一复制起点和来自自主状态的F质粒的复制起点进行了亚克隆,并作限制性内切酶酶切分析比较,没有发现两者在结构上有差异。本文的结果提示,F质粒和F′质粒在发动染色体复制中对recA基因的依赖性的不同,可能与质粒整合在染色体上的位置不同有关。The replication origin of the integrated F' plasmid was cloned by means of marker rescue. No difference in incompatibility and acridine orange sensitivity was found between the mini-F plasmid constructed from such an origin and the autonomous F' plasmid. Subcloning and comparative restriction enzyme analysis were carried out with the replication origin from tne integrated F' plasmid and that from the autonomous F plasmid. No structural difference was found between them. These results suggest that the difference for the dependence of recA gene between the F and F' plasmids dose not come from any difference in structure of their replication origin, but probably from their site of integration on the chromosome.

关 键 词：F'质粒 复制 克隆 大肠杆菌 

分 类 号：Q785[生物学—分子生物学]