苏云金芽胞杆菌治理镍污染的方法的建立  

Treatment of Nnickel Pollution with Bacillus thuringiensis

作　　者：苏新华[1] 潘洁茹 蒋捷[1] 黄天培[1] 

机构地区：[1]福建农林大学生物农药与化学生物学教育部重点实验室,福建福州350002 [2]福州市疾病预防与控制中心,福建福州350004

出　　处：《激光生物学报》2011年第5期699-702,共4页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(31071745);教育部博士点基金项目(200935151 10010);卫生部科学研究基金项目(WKJ2008-2-44);科技部农业科技成果转化资金项目(2010GB2C400212);福建省高校服务海西建设重点项目(0b08b005);福建省教育厅科技项目(JA10099);福州市科技计划项目(2009-N-43);福建省青年人才项目(2009J505165)

摘　　要：利用微生物治理重金属污染已经成为一个研究的热点,并被视为将最终替代传统的物理、化学等处理办式的一种方法。但由于一些微生物存在安全性、繁殖速度慢等问题而造成了处理效果不佳。因此,以安全性高、繁殖速度快的苏云金芽胞杆菌(Bacillus thuringiensis,简称Bt)为研究载体,寻找最适Bt的镍污染处理方法对于提高治理效果具有重要意义。研究结果表明,与直接吸附法相比,利用菌体重悬法治理Ni(Ⅱ)污染的方法更具应用前景,其最大平衡富集量达到32.29mg/g。此外,研究还发现不同时期的(营养期、孢子囊期和晶体-孢子混合期)Bt富集Ni(Ⅱ)的能力不同,营养期Bt的Ni(Ⅱ)富集量显著高于其他两个时期。The biosorption process has been studied extensively using microbial biomass as biosorbents for heavy metal removal in recent years. Biosorption of heavy metals by microbial cells has been recognized as a potential alternative to existing technologies for recovery of heavy metals from industrial waste streams. However, there are some problems about microorganism safety of and its low propagation speed, and this causes poor efficiency of its treatment. In this study, Bacillus thuringiensis （Bt） strains had been investigated for their ability to treat nickel-containing wastewater and was aimed to find the best way of treatment. The results showed that the method of bacterium-resuspension for treating nickel-containing wastewater was more prosperous than the direct-adsorption method. Its quantity of equilibrium accumulation reached 32.29 mg/g. Moreover, Bt strain in different growth stages （vegetative stage, sporulation stage and crystal stage） showed different adsorption capacity of Ni （ Ⅱ ）. The adsorption quantity of Bt vegetative cells was significantly higher than the other two stages.

关 键 词：苏云金芽胞杆菌 镍污染 镍吸附 

分 类 号：Q939.9[生物学—微生物学]