病理及生理状态下的栀子血清药物化学对比研究  

Comparative study on serum pharmacochemistry of Gardenia jasminoides under physiological and pathological status

作　　者：董婉茹[1] 丁雅光[1] 荆雷[1] 卢芳[1,2] 刘树民[1,2] 李俊行[1] 

机构地区：[1]黑龙江中医药大学中医药研究院,黑龙江哈尔滨150040 [2]黑龙江中医药大学北药基础与应用研究省部共建教育部重点实验室,黑龙江哈尔滨150040

出　　处：《中草药》2011年第11期2270-2274,共5页Chinese Traditional and Herbal Drugs

基　　金：国家重点基础研究发展计划"973"计划项目(2007CB512608)

摘　　要：目的以血清药物化学结合现代色谱技术为研究手段,阐明清热药栀子苦寒药性的药效物质基础。方法采用HPLC法,建立药材指纹图谱的色谱洗脱条件,Wistar大鼠ig给予栀子水提物,肝门静脉采血,对比大鼠在生理及苯酚诱导的热病症候状态下,血清中药物成分吸收的变化情况。结果建立了6批栀子水提物共有峰的色谱指纹图谱;确定最佳给药剂量、采血时间及血清处理方法。与生理状态下的大鼠给药组比较,热病症候状态下大鼠对栀子苷有显著的增量吸收,另有2个移行成分生成。结论大鼠在热证状态下对栀子水提物中栀子苷的增量吸收,以及血清中出现的移行成分等现象,表明这些成分有可能代表栀子苦寒药性。Objective By means of serum pharmacochemistry combined with modem chromatographic technology to elucidate the material basis for efficacy of aqueous extract from Gardeniajasminoides （AEGJ）, which has antipyretic activities with bitter-cold property. Methods Waters 2695 HPLC was employed as the detection system, based on the same chromatographic eluted condition, physiological and DNP-induced fever syndrome serum samples, collected from hepatic portal vein of rats after ig administration of AEGJ, were under determining to compare the absorptive variation of the chemical compounds. Results The chromatographic fingerprints of common peaks for six batches of AEGJ were established to ascertain the optimized administration dosage, blood collection time, and the method used to process the serum. Compared with the normal administrated group under physiological stares of rats, we found that the significant increment absorption of jasminoidin and the generation of two new migration compounds in the DNP fever syndrom model group. Conclusion The increment absorption and the migration of chemical compounds in serum of DNP fever syndrome pathological model of rats might reasonably lead us to consider these chemical compounds as symbols of the bitter-cold property of G jasminoides.

关 键 词：栀子 热病症候模型 血清药物化学 苦寒性 增量吸收 

分 类 号：R282.710.5[医药卫生—中药学]