限制酶AluI显带和CA_3/DA/DAPI染色表达的家猪染色体结构异染色质  

Expression of Constitutive Heterochromatin by Alul Restriction Endonuclease Treatment and CA_3/DA/DAPI Staining of Domestic Pig Chromosome

作　　者：赵亚力 王喜忠[1] 王子淑[1] 陈文元[1] 

机构地区：[1]四川大学生物系

出　　处：《Acta Genetica Sinica》1990年第5期360-364,共5页

基　　金：国家自然科学基金

摘　　要：采用限制酶AluI显带、CA_(?)/DA/DAPI荧光染色和常规C带技术研究了家猪染色体着丝粒结构异染色质,结果表明:着丝粒结构异染色质至少可被区分为3类,并且在染色体组内各有其特异的染色体分布。将家猪染色体DA/DAPI荧光带和限制酶AluI显带与人类染色体比较,发现家猪13—18号端着丝粒染色体显带特征与人染色体1,9、16、Y一致。提示家猪13—18号端着丝粒区结构异染色质存在与人类随体DNA相似的DNA组成。The heterogeneity of constitutive heterochromatin in two domestic pigs has been studied by restriction endonuclease Alul banding, CA2/DA/DAPI banding and C-banding techniques. The results show that the centromere constitutive heterochromatin can be subdivided into rhree types, which located in different chromosomes. It is found that there is correspondence between No. 13-18 chromosomes in pigs and No. 1,9,16,Y chromosome in human by comparing the banding patterns of Alul and CAs/DA/DAP1. Therefore, it is revealed that satellite DNAs in constitutive heterochromatin of chromosomes mentioned above are similar.

关 键 词：家猪 显带染色体 结构异染色质 

分 类 号：S828.2[农业科学—畜牧学]