肾组织水通道蛋白表达与肾病综合征水肿的关系  

Association between aquaporins expression in kidney tissue and edema of nephrotic syndrome patients

作　　者：李鹏[1] 牛建英[1] 张绮[1] 范伟峰[1] 罗丽红[1] 钱英俊[1] 张丽红[1] 顾勇[1] 

机构地区：[1]复旦大学附属上海市第五人民医院肾脏科,上海200240

出　　处：《中华肾脏病杂志》2011年第10期735-739,共5页Chinese Journal of Nephrology

基　　金：上海市闵行区科委项目（2009MHZ115）;闵行区重点学科建设（2006MHZ06）

摘　　要：目的了解肾组织局部水通道蛋白（AQP）表达与肾病综合征（NS）水肿的关系。方法NS患者分为水肿组（14例）及无水肿组（8例），以非NS患者为对照组（10例）。以免疫组织化学方法检测各组患者肾组织AQP1、2、4表达，分析其与患者水肿程度的关系。结果NS患者AQP1主要表达在近端肾小管，其中水肿组免疫组化阳性指数为0．0373±0．0110，表达量显著低于非水肿组的0．0510±0．0120和对照组的0．0574±0．0100，差异均有统计学意义；而后两组间差异无统计学意义。AQP1在各组。肾小球也有表达，水肿组阳性指数为0．0106±0．0037，显著高于非水肿组的0．0021±0．0013和对照组的0．0020±0．0012，差异均有统计学意义；而后两组间差异无统计学意义。AQP2主要表达在集合管系统，其中水肿组阳性指数为0．0498±0．0081，非水肿组为0．0370±0．0072，均显著高于对照组的0．0255±0．0103，差异均有统计学意义；而水肿组与非水肿组间差异也有统计学意义。AQP4在肾脏皮质集合管未见表达。结论不同AQP亚型在NS患者肾组织表达有差异。AQP2在NS水肿发生中起着重要作用，而AQP1的表达异常也参与水肿的发生。Objective To elucidate the association between aquaporins （AQPs） expression in kidney tissue and edema of nephrotic syndrome （NS） patients. Methods NS patients were divided into edema group （lg cases） and non-edema group （8 cases）. Ten patients without NS were used as control group. Expressions of AQP1, AQP2, and AQP4 in renal tissues of 3 groups were detected by immunohistochemistry with standard techniques and semi-quantitative analysis. Association between AQPs expression and edema was examined. Results The positive index of AQP1 expression in proximal tubules in edema group was 0.0373±0.0110, which was significantly lower as compared to non-edema group （0.0510±0.0120） and control group 0.0574±0.0100）, while the difference between non-edema and control groups was not significant. The positive index of AQP1 expression in glomerulus was 0.0106±0.0037 in edema group, which was significantly higher than that in non-edema group （0.0021±0.0013） and control group （0.0020±0.0012）, while no significant difference was found between the last two groups. AQP2 mainly localized in the collecting duct system. The positive indexes of AQP2 expression were 0.0498±0.0081, 0.0370± 0.0072 and 0.0255±0.0103 in edema group, non-edema group and control group, respectively. The differences were significant among 3 groups. AQP4 expression was not found in the renal cortex and collecting duct system. Conclusions AQPs expression is different in renal tissues of NS patients. AQP2 may play an important role in the edema of NS patients, and AQP1 may involve in the occurrence of edema.

关 键 词：肾病综合征 水肿 水通道蛋白质类 

分 类 号：R692.3[医药卫生—泌尿科学]