穿通支原体感染致IgA肾病的实验动物模型  

Experimental IgA nephropathy animal model induced by Mycoplasma penetrans infection

作　　者：姜雪[1] 许菲菲[1] 章建娜[1] 史艳玲[1] 张骥[1] 陈朝生[1] 吕吟秋[1] 孙梅[1] 

机构地区：[1]浙江省温州医学院附属第一医院肾内科,325000

出　　处：《中华肾脏病杂志》2011年第10期775-778,共4页Chinese Journal of Nephrology

基　　金：浙江省科技计划项目（2008C33029）

摘　　要：目的利用穿通支原体（Mycoplasm penetrans，Mpe）感染小鼠，制作新的IgA肾病动物模型。方法分别采用培养至对数期的Mpe菌液、SP-4培养基和PBS经尿路上行感染小鼠并联合尾静脉冲击的方法制作IgA肾病模型，与常用IgA肾病模型进行比较，观察肾脏病理改变。结果成功通过Mpe培养液经尿道上行感染小鼠制作IgA肾病模型，造模成功率100％，与常用模型组比较，IgA沉积率和荧光强度差异均无统计学意义。免疫荧光检查中，Mpe感染组不仅发现较强的IgA沉积，同时发现66．67％的小鼠肾组织存在IgG沉积。光镜检查发现，Mpe感染组系膜细胞增生和系膜基质沉积程度均明显重于PBS和SP-4培养基对照组（均P〈0．05），与常用IgA肾病模型比较，差异无统计学意义。此外Mpe感染组存在明显的肾间质单个核细胞浸润和包曼囊腔内壁层上皮细胞增生等病理改变。结论通过Mpe经尿路上行感染小鼠可以成功制作IgA肾病动物模型，为IgA。肾病病原体的研究提供了新的方向。Objective To make a new IgA nephropathy （IgAN） animal model by infecting mice with Mycoplasma penetrans （Mpe）. Methods Mice were infected through urinary tract with Mpe, Sp-4 medium or PBS, and combined with tail vein challenge to make experimental IgAN animal model. Pathologic changes were compared between new IgAN model and classical IgAN model. Results A new IgAN animal model was established successfully and the successful rate was 100%. There was no significant difference in IgA deposition rate or fluorescence intensity between our new IgAN animal model and the classical IgAN animal model.There was also IgG deposition found in 66.67% Mpe-infected mice. Light microscopy examination revealed compared with PBS or SP-4 medium control group, the proliferation of mesangial cells and the deposition of matrix were more serious in Mpe-infection group （all P〈0.05）, which was not signifcantly different with classical IgAN group. Besides, there was mononuclear cell infiltration in tubular interstitium and parietal cell proliferation in Bowman＇s capsule in Mpe-infection group. Conclusion By infecting mice with Mpe through urinary tract, IgAN animal model can be successfully made which may offer a new direetion for IgAN pathogen research.

关 键 词：肾小球肾炎 IGA 支原体 穿透 模型 动物 

分 类 号：R692.3[医药卫生—泌尿科学] R-332[医药卫生—外科学]