新型降血糖化合物G004对kkay小鼠糖脂代谢的作用  

Effect of G004 on glucose and lipid metabolism in type 2 diabetic kkay mice

作　　者：尚利娜 皋聪[2] 巫冠中[2] 张文平[3] 

机构地区：[1]南京明基医院有限公司,南京210024 [2]中国药科大学,南京210009 [3]浙江理工大学,杭州310018

出　　处：《中国新药杂志》2011年第21期2057-2062,2068,共7页Chinese Journal of New Drugs

基　　金：国家"重大新药创制"科技重大专项(2009ZX09103-094);国家自然科学基金(30973638)

摘　　要：目的:研究新型降血糖化合物G004对自发性糖尿病kkay小鼠糖脂代谢的影响。方法:采用自发性2型糖尿病kkay小鼠连续灌胃给药,测定血糖、血脂和血糖调节激素水平;采用β-TC-6胰岛素分泌细胞,测定G004对细胞分泌胰岛素的作用;制备大鼠体外灌流胰腺,放射免疫法测定灌流流出液中的胰岛素含量。结果:G004有效降低kkay小鼠空腹血糖、三酰甘油水平,并显著降低游离脂肪酸;G004促进β-TC-6细胞及体外灌流胰腺分泌胰岛素。结论:化合物G004可能通过促进胰岛素分泌改善kkay小鼠糖代谢和脂代谢。Objective： To investigate the effect of G004 on glucose and hpid metabolism in type 2 diabetic kkay mice. Methods： The effect of G004 on blood glucose, lipid and the hormones regulating blood glucose was examined in type 2 diabetic kkay mice. The insulin-secreting cell line β-TC-6 cells were used to examine its effect on stimulation of insulin secretion. Isolated rat pancreas was isolated and perfused with G004, and insulin concentrations in effluent perfusate were measured by radioimmunoassay （ RIA）. Results ： The fasting plasma glucose, triglyceride level and free fatty acids levels were effectively reduced in kkay mice treated with G004. Insulin secretion was effectively promoted by G004 from the perfused pancreas and β-TC-6 cells. Conclusion： G004 stimulates insulin secretion and ameliorates the abnormity of glucose and lipid metabolism in kkay mice.

关 键 词：KKAY小鼠 G004 脂质代谢 

分 类 号：R96[医药卫生—药理学] R977.15[医药卫生—药学]