小檗碱对早期糖尿病肾病大鼠肾组织病理变化与BMP_7蛋白及mRNA表达的影响  

Effect of berberine on renal pathology and bone morphogenetic protein-7 expressions in rats with early diabetic nephropathy

作　　者：余娜[1,2] 刘圣[2] 张小力[1,2] 陈象青[2] 唐丽琴[2] 吕飞[1,2] 

机构地区：[1]安徽中医学院药学院,合肥230038 [2]安徽医科大学附属省立医院,合肥230001

出　　处：《中国新药杂志》2011年第21期2149-2155,共7页Chinese Journal of New Drugs

摘　　要：目的:研究小檗碱(BBR)对糖尿病肾病(DN)大鼠肾组织病理变化与骨形成蛋白-7(BMP-7)蛋白及mRNA表达的影响,探讨BBR对DN大鼠早期肾脏损伤的作用及其可能机制。方法:以链脲佐菌素(STZ)复制早期DN大鼠模型,动物分为正常对照组、模型组、3个BBR(50,100,200 mg.kg-1)治疗组及依那普利(1 mg.kg-1)治疗组,灌胃给药,qd,5周后检测各组大鼠尿量、左肾重、肾重/体重比值;生化指标检测空腹血糖(FBG)、尿素氮(BUN)、血肌酐(Scr)、胰岛素(Ins)、24 h尿蛋白量(Upro)及24 h尿微量白蛋白(Um-Alb);光镜和电镜观察肾组织形态学和超微结构的改变;免疫组化技术和逆转录聚合酶链反应(RT-PCR)检测肾组织BMP-7蛋白和mRNA表达。结果:与模型组比较,BBR各治疗组DN大鼠FBG,BUN,Scr,Upro及UmAlb水平降低,Ins升高;肾组织形态学异常和超微结构病变改善;BMP-7蛋白和mRNA表达增多。结论:BBR可显著升高早期DN大鼠肾组织BMP-7蛋白及mRNA的表达,改善早期DN大鼠肾功能病变,延缓DN发展。Objective： To determine the effect of berberine （BBR） on the pathological change and expression of bone morphogenetic protein-7 （BMP-7） in the kidney of rats with early diabetic nephropathy （DN）. Methods： DN was induced by injection of streptozotocin （STZ）. The rats were intragastrically administered with BBR and enalapril once daily. After 5 weeks, the kidney weight, 24-h urinary volume, kidney/body weight ratio, fasting blood glucose （FBG） , blood urea nitrogen （BUN） , serum creatinine （Scr） , insulin （INS） , urinary protein （Upro） , and urinary microalbumin were tested. The pathological changes and ultrastructures in renal tissue were examined by optical or electron microscopy. Immunohistochemistry and RT-PCR were used to detect the expression of BMP-7 protein and mRNA in the renal tissues. Results： In BBR-treated rats, serum levels of FBG, BUN, Scr, Upro and UmAlb were decreased, while the insulin level increased. The expression of BMP-7 protein and mRNA in renal tissues was decreased. BBR ameliorated the pathological changes and uhrastructureal lesions in the kidney of DN rats. Conclusion： BBR can obviously improve renal function and increase the expression of BMP-7 in the kidney, and delay the progression of diabetic nephropathy in DN rats.

关 键 词：小檗碱 糖尿病肾病 骨形成蛋白-7 病理形态学 超微结构 

分 类 号：R977.15[医药卫生—药品]