高效液相色谱-荧光检测法测定血浆中五聚赖氨酸-β-羰基酞菁锌  

Determination of pentalysine-β-carbonylphthalocyanine zinc in mouse plasma by HPLC with fluorescence detection

作　　者：李春艳[1] 李晓静[1] 单丽[2] 黄明东[3] 

机构地区：[1]福建医科大学药学院,福州350108 [2]福建中医药大学附属第一医院,福州350004 [3]中国科学院福建物质结构研究所结构化学国家重点实验室,福州350002

出　　处：《中国新药杂志》2011年第21期2156-2159,共4页Chinese Journal of New Drugs

基　　金：国家自然科学杰出青年基金(30625011);国家高技术研究发展计划(863计划)(2006AA02A313)

摘　　要：目的:建立高效液相色谱-荧光检测法测定小鼠血浆中五聚赖氨酸-β-羰基酞菁锌[ZnPc-(Lys)5]的浓度。方法:血浆样品经预处理后采用CNW C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(分别含有0.05%三氟乙酸),流速为1.0 mL.min-1,荧光检测器激发波长为610 nm,发射波长为690 nm,柱温为25℃。结果:在2～500 nmol.L-1检测浓度范围内线性良好(r=0.999 0),检出限为0.5 nmol.L-1,提取回收率为86.99%～88.80%,相对回收率为96.20%～101.76%,日内和日间RSD均低于10%。结论:该方法简便、快速、准确、重复性好,可用于血浆中ZnPc-(Lys)5浓度的测定。Objective： To establish an HPLC method with fluorescence detection for determining the concentration of pentalysine-β-carbonylphthalocyanine zinc [ ZnPc-（Lys）5] in mouse plasma. Methods： After a sim- ple pretreatment, ZnPc-（Lys）5 was separated on a CNW CIS column （250 mm ×4.6 ram, 5 μm） and detected by fluorescence detector. The wavelengths were set at Ex = 610 nm and Em= 690 nm. The mobile phase was consisted of methanol-water （containing 0.05% TFA） at a flow rate of 1 mL· min-l. The column temperature was set at 25 ℃. Results： The concentrations of ZnPc-（Lys）5 showed good linearity in the range of 2 -500 nmol· L-1（r = 0. 999 0）. The lowest limit of quantification was 0.5 nmol. L-1 The extraction recoveries and relative recoveries were in the ranges of 86.99% - 88.80% and 96.20% -101.76% , respectively. The intra- and inter-day preeisions were less than 10%. Conclusion： This method is simple, rapid, accurate and reproducible for determining the concentration of ZnPc-（Lys） 5 in mouse plasma.

关 键 词：高效液相色谱法 荧光检测 血浆 光敏剂 五聚赖氨酸-β-羰基酞菁锌 

分 类 号：R969.11[医药卫生—药理学]