耐药大肠埃希菌β-内酰胺类、氨基糖苷类获得性耐药基因与可移动遗传元件研究  

Investigation of acquired resistance genes to β-lactams,aminoglycosides and mobile genetic elements in multidrug-resistant Escherichia coli

作　　者：张建明[1] 万长标[1] 钱玮和[1] 程建平[1] 金跃[1] 

机构地区：[1]淮安市第二人民医院检验科,江苏淮安223002

出　　处：《中华医院感染学杂志》2011年第22期4658-4661,共4页Chinese Journal of Nosocomiology

摘　　要：目的调查多药耐药大肠埃希菌β-内酰胺类、氨基糖苷类获得性耐药基因与可移动遗传元件的存在情况。方法收集医院2007年1-12月患者标本中分离的20株多药耐药大肠埃希菌,采用聚合酶链反应(PCR)的方法,分析42种β-内酰胺类、氨基糖苷类获得性耐药基因以及10种可移动遗传元件的遗传标记;并对上述检测结果做样本聚类分析。结果 20株多药耐药大肠埃希菌检出β-内酰胺类获得性耐药基因TEM-1及CTX-M-55,检出率为80.0%、50.0%,氨基糖苷类获得性耐药基因aac(3)-Ⅱ、aac(6′)-Ⅰb、ant(3″)-Ⅰ、aadA5、aph(3′)-Ⅰ,检出率分别为45.0%、5.0%、10.0%、45.0%、5.0%,可移动遗传元件检出intⅠ1、tnp513、IS26、ISEcp1、traA、trbC,检出率分别为55.0%、25.0%、90.0%、65.0%、80.0%、75.0%;其他39种基因未检出。结论 20株多药耐药大肠埃希菌耐β-内酰胺类、氨基糖苷类药物与细菌产2种β-内酰胺类获得性耐药基因、5种氨基糖苷类获得性耐药基因相关;可移动遗传元件的水平转移使细菌的耐药性在同种细菌菌株之间甚至不同种细菌菌株之间得以快速传播;样本聚类分析提示,该组多药耐药大肠埃希菌尚无克隆传播。OBJECTIVE To investigate the distribution of acquired resistance genes to β-lactams,aminoglycosides and mobile genetic elements in multidrug-resistant Escherichia coli（E.coli）. METHODS From Jan 2007 to Dec 2007,20 strains of E.coli were collected from The Second People′s Hospital of Huaian.Then,42 kinds of acquired resistance genes to β-lactams,aminoglycosides and 10 kinds of genetic markers of mobile genetic elements were analyzed by PCR.In addition,sample cluster analysis was performed. RESULTS In 20 strains of multidrug-resistant E.coli,acquired resistance genes to β-lactams： TEM-1,CTX-M-55,acquired resistance genes to aminoglycosides： aac（3）-Ⅱ,aac（6′）-Ⅰb,ant（3″）-Ⅰ,aadA5,aph（3′）-Ⅰ,genetic markers of mobile genetic elements： intⅠ1,tnp513,IS26,ISEcp1,traA,trbC were detected.However,the rest 39 kinds of genes couldn＇t be detected. CONCLUSION Two kinds of acquired resistance genes to β-lactams and 5 kinds of acquired resistance genes to aminoglycosides might play a role in resistance to β-lactams and aminoglycosides in this 20 strains of multidrug-resistant E.coli.And horizontal transfer of mobile genetic elements brought rapid spread of resistance of bacterial pathogens,not only among the same kinds of pathogens,but among the different ones.In addition,sample cluster analysis shows that no clone transmission was found in this group of E.coli.

关 键 词：大肠埃希菌 Β-内酰胺类 氨基糖苷类 获得性耐药基因 可移动遗传元件 

分 类 号：R378.21[医药卫生—病原生物学]