磁珠法检测抗酸杆菌的方法建立与临床应用  

Establishment and clinical application of TB-Beads in testing acid-fast bacilli′s

作　　者：刘君[1] 裴豪[1] 陈燕燕[1] 蒯守刚[1] 陈丽艳 王旭[1] 

机构地区：[1]无锡市传染病医院检验科,江苏无锡214005 [2]无锡市河埒街道社区卫生服务中心,江苏无锡214072

出　　处：《中华医院感染学杂志》2011年第22期4861-4863,共3页Chinese Journal of Nosocomiology

基　　金："十一五"国家科技重大专项(2009ZX10004-313)

摘　　要：目的运用磁珠法检测痰液标本中的抗酸杆菌,探讨该技术在抗酸杆菌诊断中的临床应用价值。方法采用磁珠法检测102份痰液标本中的抗酸杆菌,并与离心沉淀法平行检测结果相比较。结果同一份标本经磁珠法处理后镜下视野更清晰,抗酸杆菌和背景对比更鲜明,102份痰液标本中离心沉淀法检测的阳性率为47.1%,磁珠法检测的阳性率为51.0%,54份离心沉淀法检测阴性的标本用磁珠法检测有4份阳性,平行检测同一份标本,磁珠法检测出的阳性等级更高,差异有统计学意义(P<0.01)。结论磁珠检测方法操作简便、快速,其阳性率高,浓缩效果明显,适合结核病检测实验室的推广。OBJECTIVE To detect the acid-fast bacilli in the sputum samples by TB-Beads and discuss its clinic application. METHODS A total of 102 sputum samples were obtained.Then the acid-fast bacilli were detected and the results were compared with those from the group by centrifugation method. RESULTS Compared with centrifugation method,the more clearer arthroscopic characteristics was found following the TB-Beads,the acid-fast bacilli also looked brighter.In the 102 sputum samples,the positive rate of centrifugation method was 47.1%,while the positive rate of TB-Beads was 51.0%,54 samples were negative by centrifugation while 4 samples in these 54 samples were positive through the TB-Beads.The positive grades were higher from the TB-Beads in the same sample,the difference showed statistical significance（P〈0.01）. CONCLUSION The TB-Beads shows simple and fast operation,higher positive rate,palpable and effective concentration,and is suitable for tuberculosis diagnosis in the lab.

关 键 词：磁珠法 离心沉淀法 结核分枝杆菌 

分 类 号：R378.91[医药卫生—病原生物学]