杏杂合位点共显性标记的分离方式及连锁图谱构建  

Segregation Type of Heterozygous Loci in F1 and Construction of Molecular Markers Linkage Map in Apricot

作　　者：章秋平[1] 刘威生[1] 刘宁[1] 张玉萍[1] 刘硕[1] 魏潇[1] 刘有春[1] 

机构地区：[1]辽宁省果树科学研究所,国家果树种质熊岳李杏圃,辽宁熊岳115009

出　　处：《园艺学报》2011年第10期1983-1990,共8页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31071767);公益性行业(农业)科研专项(201003058);农业部作物种质资源保护项目(NB2010-2130135-03)

摘　　要：以‘串枝红’ב金太阳’杏的F1代为材料,在分析共显性标记分离方式的基础上利用简单重复序列(SSR)和相关序列扩增多态性(SRAP)标记,构建了两张杏分子连锁图谱。‘串枝红’图谱共14个连锁群,包含37个SSR标记和32个SRAP标记,连锁群总长度为1086.6cM,每连锁群平均长度77.6cM,标记间平均连锁距离为19.7cM。‘金太阳’连锁图谱共7个连锁群,包括26个SSR标记和14个SRAP标记,连锁群总长度756.7cM,每个连锁群平均长度为108.1cM,标记间平均距离为22.9cM。分析了图谱构建过程中不同共显性标记在F1代分离类型中的利用。Genetic linkage maps for two apricot cultivars have been constructed using simple sequence repea（tSSR）and sequence-related amplified polymorphism（SRAP）markers in 94 F1 individuals from the cross Chuanzhihong×Golden-sun.A total of 37 SSR and 32 SRAP markers were placed into 14 linkage groups on the Chuanzhihong map,defining 1 086.6 cM of the total map-length,and the average length of group was 77.6 cM.The average distance between adjacent markers was 19.7 cM.The male parent map of Golden-sun consisted of 26 SSR and 14 SRAP markers could be assigned to 7 linkage groups,defining 756.7 cM of the total map-distance.The average distance of group was 108.1 cM,with an average interval of 22.9 cM between adjacent markers.Segregation type of heterozygous loci in F1 was discussed in constructing map of apricot.

关 键 词：杏 杂合位点 分子标记 分离类型 连锁图谱 

分 类 号：S662.2[农业科学—果树学]