脉花党参中党参炔苷含量的高效液相色谱测定  

Determination of Lobetyolin in Codonopsis nervosa by High Performance Liquid Chromatography

作　　者：谢光波[1] 吴超[1] 范强[2] 周先礼[2] 格桑索朗 

机构地区：[1]电子科技大学生命科学与技术学院,四川成都610054 [2]西南交通大学生命科学与工程学院,四川成都610031 [3]西藏自治区食品药品检验所,西藏拉萨850000

出　　处：《时珍国医国药》2011年第6期1321-1322,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家"十一五"科技支撑计划项目(No.2007BAI31B02)

摘　　要：目的测定脉花党参中党参炔苷的含量。方法采用高效液相色谱法;Sh im-pack CLC-ODS色谱柱,波长267nm,流动相：乙腈-水（17∶83）,流速1 m l/min。结果脉花党参中党参炔苷在0.075~6.25μg线性关系良好（R2=0.998 9）。平均回收率为99.1%,RSD=2.3%。结论以高效液相色谱法测定脉花党参中党参炔苷的含量,操作简便,专属性好。Objective To determine lobetyolin content in Condonopsis nervosa by HPLC.Methods Shim-pack CLC-ODS（150 mm × 4.6 mm,5 μm） was used with acetonitrile-water（17∶83） as the mobile phase and UV detection was wavelength at 267 nm.Results The linear range was within the range of 0.076 ~ 6.25 μg（R2 = 0.998 9）,the average recovery was 99.1%,and RSD was 2.3%.Conclusion The method is reliable,accurate and very suitable for the determination of lobetyolin in Condonopsis nervosa.

关 键 词：脉花党参 党参炔苷 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]