辛通畅络法干预局灶节段性肾小球硬化大鼠细胞外基质积聚的实验研究  

Effects of Dredging Vessels by Xintong on Mesangial Matrix Proliferation in FSGS Rat Glomerulus

作　　者：窦一田[1] 任桐[2] 曹式丽[2] 

机构地区：[1]天津中医药大学,2009级博士研究生天津300193 [2]天津中医药大学第一附属医院,天津300192

出　　处：《时珍国医国药》2011年第9期2070-2072,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30772808)

摘　　要：目的观察辛通畅络法对局灶节段性肾小球硬化(FSGS)大鼠肾小球细胞外基质(ECM)增殖的影响。方法阿霉素注射法诱发FSGS型阿霉素肾病大鼠模型,以辛通畅络法的载体中药复方肾苏Ⅱ为治疗药物,贝那普利为对照药物进行干预。于治疗第12周末,切取肾脏进行病理学观察及免疫组化检测,观察肾小球硬化、基质积聚程度及其主要成分表达。结果病理学观察及免疫组化检测显示,中、西药组均出现肾小球硬化进展减缓,基质增殖抑制,FN、LN及ColⅣ表达降低,且中药组具干预优势。结论辛通畅络法可通过抑制FSGS大鼠肾小球ECM的过度积聚,延缓其肾小球硬化进展。Objective To observe the effects of Dredging Vessels by Xintong on mesangial matrix proliferation in the FSGS rat glomerulus. Methods Rat focal segmental glomerulosclerosis animal model was induced by injecting doxo-rubicin hydrochloride.Test and observe the changes of pathomorphology and expression of fibronectin,collagenⅣ and laminin in glomerulus. Results ShensuⅡ could alleviate FSGS rat pathomorphological changes and slow the progresses of glomerulosclerosis by inhibiting the expression of fibronectin,collagenⅣ and laminin in the glomerulus. Conclusion Focal segmental glomerulosclerosis can be treated by ShensuⅡ and the therapy mechanism is related to reducing mesangial matrix accumulation.

关 键 词：局灶节段性肾小球硬化 辛通畅络法 肾苏Ⅱ 细胞外基质 

分 类 号：R285.5[医药卫生—中药学]