扶正解毒颗粒对染镍大鼠肾组织及尿单核细胞趋化蛋白-1水平的影响  

Effect of Fuzheng Jiedu Granula on the Expression of MCP-1 in Rats Exposed to Nickel

作　　者：吕晓云[1] 孙应彪[2] 程卫东[1] 朱玉真[2] 赵健雄[1] 

机构地区：[1]兰州大学中西医结合研究所,甘肃兰州730000 [2]兰州大学公共卫生学院,甘肃兰州730000

出　　处：《时珍国医国药》2011年第9期2219-2221,共3页Lishizhen Medicine and Materia Medica Research

基　　金：甘肃省自然科学研究基金(No.096RJZA060);兰州大学医学科研基金资助项目(No.LZUYX200805)

摘　　要：目的研究中药扶正解毒颗粒(FJG)对硫酸镍(NiSO4)暴露肾组织单核细胞趋化蛋白-1(MCP-1)mRNA表达的影响。方法 Wistar大鼠NiSO4(2.5 mg.kg-1.d-1)染毒造模,FJG灌胃处理,分别采用酶联免疫吸附试验(ELISA)法和双缩脲比色法检测尿MCP-1和24 h尿蛋白含量;采用实时荧光定量RT-PCR检测肾组织MCP-1 mRNA表达的变化。结果 NiSO4组肾细胞MCP-1mRNA表达增强,尿MCP-1和24 h尿白蛋白含量增加;FJG组MCP-1 mRNA表达水平明显低于NiSO4组(RSD分别为4.3%,6.9%,7.4%),FJG组尿MCP-1和24 h尿白蛋白含量明显低于NiSO4组(P<0.05和P<0.01)。结论 MCP-1mRNA表达增强、尿MCP-1含量增加与镍性肾损伤存在一定关联,FJG干预镍肾毒性的作用可能与下调MCP-1mRNA表达及降低尿MCP-1水平有关。Objective To study the effect of Fuzheng Jiedu Granula（FJG） on the expression of monocyte chemoattractant protein-1（MCP-1） mRNA and urine MCP-1 level in rats exposed to nickel sulfate（NiSO4）. Methods Nephrotoxicity was induced in Wistar rats by intraperitoneal injection of NiSO4（2.5 mg·kg-1·d-1） and FJG（5,10,20 mg·kg-1·d-1） was administered by gavage.MCP-1mRNA expression was detected by real-time fluorescence quantitative RT-PCR（FQ RT-PCR） assay.The levels of proteinuria and urine MCP-1 were evaluated with biuret colorimetric assay and enzyme-linked immunosorbent（ELISA） assay,respectively. Results In NiSO4 group,the expression of MCP-1mRNA in renal cells was enhanced and urine MCP-1 level was increased.Compared with NiSO4 group,the expression of MCP-1 was down-regulated（RSD=4.3%,RSD=6.9% and RSD=7.4%） and the levels of proteinuria and urine MCP-1 in FJG groups were decreased obviously（P0.05,P0.01）. Conclusion MCP-1 expression and urine MCP-1 enhancement can be correlated with nickel-induced nephrotoxicity.By down-regulating the expression of MCP-1 and urine MCP-1 level,FJG can inhibit kidney injury induced by nickel.

关 键 词：扶正解毒颗粒 硫酸镍 肾脏 单核细胞趋化蛋白-1(MCP-1) 实时荧光定量RT-PCR 

分 类 号：R285.5[医药卫生—中药学]