硼等4种元素对甘草酸生物合成关键酶基因表达的RT-PCR分析  

RT-PCR Analysis on the Differential Expression of the Key Enzyme Genes Involved in Glycyrrhizic Acid Synthetic Metabolic Pathway under B、Mn、Zn and Mo Elements Treatment

作　　者：梁新华[1] 栾维江[2] 梁军 张风侠[1,4] 

机构地区：[1]宁夏大学生命科学学院,宁夏银川750021 [2]天津师范大学生命科学学院细胞遗传与分子调控重点实验室,天津300387 [3]宁夏回族自治区吴忠市盐环定扬水管理处,751100 [4]宁夏回族自治区银川市永宁县望洪中学,750100

出　　处：《时珍国医国药》2011年第10期2351-2353,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30600806)

摘　　要：目的研究B、Mn、Zn和Mo等4种微量元素对甘草酸生物合成关键酶鲨烯合成酶(SQS)和β-香树脂醇合成酶(β-AS)基因表达的影响。方法设置上述4种微量元素0,0.05%,0.1%,0.15%和0.3%5个不同浓度水培乌拉尔甘草幼苗,9天后,取其根部半定量PCR检测。结果能够促进SQS基因表达的是高和极高浓度的Zn元素(0.15%和0.3%)和中、高和极高浓度的Mo元素(0.1%,0.15%和0.3%);Zn元素所有处理浓度均可明显提高β-AS基因的表达,随着Mo元素处理浓度的提高,β-AS基因的表达量增加。结论 Zn和Mo两种元素对乌拉尔甘草甘草酸生物合成关键酶基因表达有明显的促进作用。ObjectiveTo investigate the effects of Boron,Manqanese,Zinc and Molybdenum treatments on the differential expression of the key enzyme（SQS and β-AS） genes involved in glycyrrhizic acid synthetic metabolic pathway in Glycyrrhiza uralensis Fisch.seedling. MethodsFive different concentrations（0,0.05%,0.1%,0.15% and 0.3%） were used in solution culture experiment,and the roots of Glycyrrhiza uralensis Fisch.seedling were harvested separately after 9 days to investigated SQS and β-AS genes by RT-PCR. ResultsThe expression of SQS and β-AS gene were significantly higher with applying Znic of three concentrations（0.05%,0.1% and 0.15%） and Molybdenum of medium to high concentrations（0.1% and 0.15%） than the other treatments. ConclusionZinc and Molybdenum can increase the expression level of SQS and β-AS genes involved in glycyrrhizic acid synthetic metabolic pathway in Glycyrrhiza uralensis Fisch.seedling.

关 键 词：甘草 甘草酸 关键酶 基因表达 微量元素 半定量PCR 

分 类 号：S567[农业科学—中草药栽培]