脑得生软胶囊中三七皂苷R_1、人参皂苷Rg_1和人参皂苷Rb_1含量的HPLC-ELSD法测定  

Determination of Notoginsenoside R_1,Ninsenoside Rg_1 and Ginsenoside Rb_1 in Naodesheng Soft Capsules by HPLC-ELSD

作　　者：胡迪[1] 樊文娟[2] 邵贝贝[2] 吴慧[2] 王光忠[2] 

机构地区：[1]湖北省中医院,湖北武汉430061 [2]湖北中医药大学药学院,湖北武汉430065

出　　处：《时珍国医国药》2011年第10期2378-2379,共2页Lishizhen Medicine and Materia Medica Research

基　　金：湖北省自然科学基金(No.2008CDB234)

摘　　要：目的建立脑得生软胶囊中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的含量测定方法。方法采用高效液相色谱-蒸发光散射检测法(HPLC-ELSD),色谱柱Hypersil ODS-C18(4.6 mm×250 mm,5μm);流动相乙腈-水,梯度洗脱(0→20 min,乙腈20%→40%);流速1.0 ml.min-1;柱温25℃;检测器参数:漂移管温度40℃,载气压力3.5 kPa。结果三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的线性范围分别为0.150～3.00μg(r=0.999 1)、0.753～15.06μg(r=0.999 6)和0.755～15.10μg(r=0.999 5),其回收率分别为98.41%(RSD=1.4%)、98.91%(RSD=1.2%)和99.34%(RSD=1.5%)。结论该方法简便、灵敏、重现性好,可作为脑得生软胶囊的质量控制方法。ObjectiveTo develop an HPLC-ELSD method for the determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rbl in Naodesheng soft capsules. MethodsAlltima C18 chromatography column was used with acetonitrile-water（0→20min,acetonitrile20%→40%） as mobile phase.The flow rate of mobile phase was 1.0 ml·min-1,the tube temperature of the detector was 40℃,and the press of pure air was 3.5 kPa. ResultsThe linear ranges were 0.150～3.00 μg（r=0.999 1）for notoginsenoside R1,0.753～15.06 μg（r=0.999 6）for ginsenoside Rg1 and 0.755～15.10 μg（r=0.999 5）for ginsenoside Rb1.The average recoveries were 98.41% with the corresponding RSD 1.4% for notoginsenoside R1,98.91% with corresponding RSD 1.2% for ginsenoside Rg1 and 99.34% with corresponding RSD 1.5% for ginsenoside Rbl,respectively. ConclusionThe method is simple,sensitive and reproducible and can be used for quality control of Naodesheng soft capsules.

关 键 词：脑得生软胶囊 高效液相色谱-蒸发光散射检测法 三七皂苷R1 人参皂苷Rg1 人参皂苷Rb1 

分 类 号：R284.2[医药卫生—中药学]