桑树寄主的4种桑寄生科药用植物槲皮素含量测定  

Determination of Quarcetin from Four Kinds of Herba Taxilli Parasiticed in Mulberry

作　　者：朱开昕 张协君 赵明惠 裴河欢 苏本伟 李永华 

机构地区：[1]广西钦州市中医医院,535000 [2]广西钦州市中医药研究所,535000

出　　处：《时珍国医国药》2011年第10期2395-2397,共3页Lishizhen Medicine and Materia Medica Research

基　　金：广西壮族自治区科学研究与技术开发计划(No.0992003B-30);广西壮族自治区自然科学基金(No.2010GXNSFA013264)

摘　　要：目的对同以桑树为寄主4种桑寄生科药用植物槲皮素含量进行测定,为扩大桑寄生药材来源的可能性提供科学的理论依据。方法采用RP-HPLC法对4种桑寄生科药用植物槲皮素含量进行测定,并与桑寄生药材对照品槲皮素含量比较,槲皮素采用甲醇-盐酸(4∶1)回流提取,用Agilent Eclipse XDB-C18柱,甲醇-0.05%磷酸溶液(47∶53)为流动相,流速1.0ml/min,检测波长254 nm。结果槲皮素的线性范围0.41～6.56 mg(r=0.999 9),平均回收率为99.25%,4种桑寄生科药用植物槲皮素含量分别为:三色鞘花0 mg/g,小红花寄生8.01 mg/g,红花寄生7.82 mg/g,广寄生5.27 mg/g。结论 4种药用植物除三色鞘花检测不到槲皮素,不能作为桑寄生药用外,小红花寄生和红花寄生的槲皮素含量较高,提示小红花寄生和红花寄生亦可作桑寄生药材使用。ObjectiveTo determine the content of quercetin in four kinds of Herba Taxilli parasiticed in Mulberry and provide the scientific basis for the possibility of expanding the source of Herba Taxilli. MethodsThe content of quercetin in four kinds of Herba Taxilli was determined by RP-HPLC and compared with the content of quercetin in reference substance of Herba Taxilli.Quercetin was refluxing extracted using methanol-hydrochloride（4：1）.The analytical column was Agilent TC-C18,the mobile phase was methanol-0.05%phosphate（47：53）,the flow rate was 1.0 ml/min,the detection wavelength was 254 nm. ResultsThe linear range of quercetin was within the range of 0.41～6.56mg（r=0.9999） and the average recovery rate was 99.25%.The content of quercetin in four kinds of Herba Taxilli was：0mg/g（Macrosolen tricolor（Leemnte）Danse）,8.01 mg/g（Scurrula parasitica var gaciliflora（Wall.exDC.）H.S.Kiu）,7.82mg/g（Scurrula parasitica L.）,5.27mg/g（Taxillus chinensis（DC.） Danser）. ConclusionMacrosolen tricolor（Lecomte） Danser can not be used as medicine because the quercetin can not be determined.The content of quercetin is high in Scurrula parasitica L.and Scurrula parasitica var gaciliflora（Wall.exDC.）H.S.Kiu which can used as medicine.

关 键 词：广寄生 红花寄生 小红花寄生 三色鞘花 桑树 槲皮素 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]