唐古特白刺红色素毒理学安全性评价  

Safety Assessment of Nitraria tangutorum Bobr.Pigment

作　　者：赵晓辉[1] 王萍[1] 陶燕铎[1] 邵贇[1] 梅丽娟[1] 

机构地区：[1]中国科学院西北高原生物研究所,青海西宁810008

出　　处：《时珍国医国药》2011年第10期2426-2428,共3页Lishizhen Medicine and Materia Medica Research

基　　金：科技部国家科技支撑计划(No.2007BAI45B00)

摘　　要：目的对唐古特白刺红色素毒理学安全性进行评价。方法清洁级昆明种小鼠、SD大鼠以唐古特白刺红色素各按最大耐受量(MTD)等体积灌胃,记录中毒表现及死亡情况;采用平板掺入法;分加与不加S-9混合液两种;选清洁级昆明种小鼠,取胸骨骨髓制片;检查骨髓嗜多染红细胞微核发生率;选雄性小鼠;取附睾制片;查小鼠精子畸形率;90 d喂养实验,观察生长发育、食物利用率、血液学检查、生化检查和组织病理检测结。结果雌雄性小、大鼠均未见明显的中毒症状及死亡;在加与不加S-9的情况下,均未呈现致突变作用;小鼠微核实验、小鼠精子畸形实验均呈阴性;90 d喂养大鼠,全部存活,且发育良好,选定各指标均未见异常。结论唐古特白刺红色素对两种性别的大、小鼠的MTD均大于30 000 mg/(kg.bw),说明该产品属实际无毒物;三项致突变实验结果阴性;大鼠90d喂养实验各项指标均无明显异常。ObjectiveTo assess the safety of Nitraria tangutorum Bobr.pigment. MethodsThe experimental animals were Kun ming mice and SD rats.Mice and rats were gastrically infused with the same volume of Mitrarica tangutirum Bobr.pigment MTD.Toxicity and death status were recorded.Flat plate incorporating method was used with 2 kinds of mixture solutions which were added S-9 or not.The sternal bone marrow of clean Kunming mice was isolated and fixed and the living rate of mouse bone marrow polychromatic erythrocyte micronucleus test was checked.The epididymis of the double sides in female clean Kunming mice was cut into slices and the rate of sperm teratogenicity was observed.The growth and development,food utilization rate,the changes of blood and biochemistry were obseved after the animals were fed for 30 days. ResultsNo obvious poisoned symptom and death were observed.Without adding S-9 condition,the mutation effect of all the animals didn＇t appear.The micronucleus test and the sperm teratogenicity test in mice were negative.All rats survived after feeding for 90 days.Animal growth in each group was good,and no abnormal phenomenon was observed. ConclusionThe MTD of Nitraria tangutorum Bobr.pigment mice and rats is greater than 30 000 mg/（kg.bw）.It is concluded that the product has no toxicity.The result of mutagenic test is negative and no abnormal phenomenon is observed after feeding for 90 days.

关 键 词：唐古特白刺红色素 安全性评价 

分 类 号：R285.5[医药卫生—中药学]