半枝莲黄酮提取物、野黄芩苷及洛伐他汀对细胞炎症模型中细胞因子IL-1β、IL-6和TNF-α基因表达及含量的影响  

Effects of the Total Flavones from Scutellaria barbata D.Don,Lovastatin and scutellarin on Gene Expression and Contents of Cytokine IL-1β,IL-6 and TNF-α in Cell Inflammatory Model

作　　者：刘俊[1] 张晨晨[1] 方圆[1] 唐宁[1] 卞卡[1] 

机构地区：[1]上海中医药大学穆拉德中药现代化研究中心,上海201203

出　　处：《时珍国医国药》2011年第10期2432-2434,共3页Lishizhen Medicine and Materia Medica Research

基　　金：上海市教育委员会科研创新项目(No.09YZ116);上海高校选拔培养优秀青年教师科研专项基金资助项目(No.SZY07026)

摘　　要：目的构建巨噬细胞炎症模型,比较研究半枝莲黄酮、洛伐他丁及野黄芩苷对细胞因子IL-1β、IL-6和TNF-α基因表达情况及含量的影响。方法培养巨噬细胞RAW264.7,利用脂多糖构建细胞炎症模型,并在不同时间收集细胞或细胞上清液,通过RT-PCR与ELISA法分别检测基因的表达情况及含量变化。结果半枝莲黄酮可以有效降低IL-6和TNF-α基因的表达,并且能够降低IL-6和TNF-α的含量,其中以IL-6最为显著,其抗炎作用要远远好于单体药物。结论半枝莲黄酮可以通过有效降低细胞因子基因的表达及其含量来挥发其抗炎作用。ObjectiveTo observe the effects of the total flavones from Scutellaria barbata D.Don,lovastatin and scutellarin on gene expression and contents of cytokine IL-1β,IL-6 and TNF-α in LPS-induced inflammatory macrophage model. MethodsInflammatory model was constructed with LPS through cell culture with macrophage RAW 264.7.Cells and supernatant were collected at different times.Gene expression and cytokine concentrations were detected with the methods of RT-PCR and ELISA. ResultsCompared with monomer lovastatin and scutellarin,the total flavones from Sculellaria barbata D.Don could effectively decrease gene expression and cytokine concentrations of IL-6 and TNF-α,especially for IL-6. ConclusionThe total flavones from Scutellaria barbata D.Don can effectively exert its anti-inflammatory effects through decreasing gene expression and contents of cytokines.

关 键 词：半枝莲 洛伐他汀 野黄芩苷 炎症 巨噬细胞 

分 类 号：R285.5[医药卫生—中药学]